Brogan M D, Shanahan F, Oliver M, Stevens R H, Targan S R
Department of Medicine, UCLA School of Medicine 90024.
J Clin Lab Immunol. 1987 Oct;24(2):69-74.
Inflammatory bowel disease patients with mild to inactive disease were studied for their ability to generate pokeweed mitogen responsive anti-tetanus toxoid IgG producing B cells in the peripheral blood 21 days following in vivo tetanus toxoid booster immunization. Compared to normals and disease controls, patients with inflammatory bowel disease had significantly impaired in vitro production of anti-tetanus toxoid antibody during an 8 day pokeweed mitogen stimulated culture period. There was poor correlation between the ratio of helper to suppressor T cells in the peripheral blood and pokeweed mitogen stimulated antibody production. Likewise, there was little correlation between in vitro antibody production and peripheral blood natural killer cell cytotoxic activity. Culture of the patients B cells with normal T cells failed to improve antibody production in vitro. These results indicate that many inflammatory bowel disease patients have an impaired humoral immune response to tetanus toxoid booster immunization. This impaired immune response may be due to an inability to generate B cell precursors of anti-tetanus toxoid IgG producing B cells rather than to abnormal circulating helper or suppressor T cell activity or natural killer cell regulatory activity.
对患有轻至非活动性疾病的炎症性肠病患者进行了研究，观察他们在体内破伤风类毒素加强免疫21天后，外周血中产生对商陆有丝分裂原反应性抗破伤风类毒素IgG的B细胞的能力。与正常人和疾病对照组相比，炎症性肠病患者在8天的商陆有丝分裂原刺激培养期内，体外抗破伤风类毒素抗体的产生明显受损。外周血中辅助性T细胞与抑制性T细胞的比例与商陆有丝分裂原刺激的抗体产生之间相关性较差。同样，体外抗体产生与外周血自然杀伤细胞细胞毒性活性之间也几乎没有相关性。将患者的B细胞与正常T细胞一起培养并不能改善体外抗体产生。这些结果表明，许多炎症性肠病患者对破伤风类毒素加强免疫的体液免疫反应受损。这种免疫反应受损可能是由于无法产生抗破伤风类毒素IgG产生B细胞的B细胞前体，而不是由于循环中辅助性或抑制性T细胞活性异常或自然杀伤细胞调节活性异常。
