Bouchard A, Watters T A, Wu S, Parmley W W, Stone R D, Botvinick E, Sievers R, Jasmin G, Wikman-Coffelt J
Department of Medicine, University of California, San Francisco 94143.
J Mol Cell Cardiol. 1987 Oct;19(10):1011-23. doi: 10.1016/s0022-2828(87)80573-4.
The effects of a sudden decrease in coronary perfusion pressure from 140 to 0 cmH2O for a 10-second interval were analyzed in normal and cardiomyopathic hamster hearts to determine whether cardiomyopathy would affect the relationship between altered coronary perfusion pressure and left ventricular geometry, wall thickness, myocardial hydrodynamics, and hemodynamics. In normal hamsters, an acute reduction in coronary perfusion pressure resulted in a decrease in left ventricular short axis epicardial cross-sectional area, base to apex length, diastolic wall thickness, myocardial water content and developed pressure. In cardiomyopathic hamsters all results induced by lowering the hydrostatic pressure of the perfusing medium were the same except that diastolic wall thickness failed to decline, indicating a decrease in intramyocardial elasticity in dilated cardiomyopathy. In parallel studies, hearts were freeze clamped at end-diastole and high energy phosphates and energy metabolites analyzed. In both normal and cardiomyopathic hamsters no significant changes were observed in ATP, PCr, or Pi levels at 10 s following the decrease in perfusion pressure. However, during the abrupt decrease in coronary perfusion pressure adenosine increased and cAMP decreased in both groups of animals. The erectile effect of altered coronary perfusion pressure is partially attenuated in the cardiomyopathic hamster in which no change in diastolic wall thickness occurs during an abrupt change in the hydrodynamics of the heart.
在正常和患心肌病的仓鼠心脏中，分析了冠状动脉灌注压在10秒内从140厘米水柱突然降至0厘米水柱的影响，以确定心肌病是否会影响冠状动脉灌注压改变与左心室几何形状、壁厚度、心肌流体动力学和血液动力学之间的关系。在正常仓鼠中，冠状动脉灌注压的急性降低导致左心室短轴心外膜横截面积、心底到心尖长度、舒张期壁厚度、心肌含水量和发育压力降低。在患心肌病的仓鼠中，降低灌注介质静水压所诱导的所有结果均相同，只是舒张期壁厚度没有下降，这表明扩张型心肌病中心肌内弹性降低。在平行研究中，在舒张末期将心脏冷冻钳夹，并分析高能磷酸盐和能量代谢物。在灌注压降低10秒后，正常和患心肌病的仓鼠的ATP、磷酸肌酸或无机磷水平均未观察到显著变化。然而，在冠状动脉灌注压突然降低期间，两组动物的腺苷均增加，而环磷酸腺苷均减少。在患心肌病的仓鼠中，冠状动脉灌注压改变的勃起效应部分减弱，在心脏流体动力学突然变化期间，其舒张期壁厚度没有变化。
