成人慢性免疫性血小板减少性紫癜患者血小板中凋亡蛋白的初步研究。

Preliminary Study on Apoptotic Proteins in Platelet from Adult Patients with Chronic Immune Thrombocytopenic Purpura.

机构信息

Division of Urological Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou, China.

Department of Clinical Laboratory Medicine, The Second Affiliated Hospital of Shantou University Medical College, Shantou, China.

出版信息

Acta Haematol. 2022;145(3):318-325. doi: 10.1159/000517812. Epub 2021 Aug 10.

DOI:10.1159/000517812

PMID:34375974

Abstract

BACKGROUND

Adult chronic idiopathic thrombocytopenic purpura (ITP) is a chronic and usually lifelong hemorrhagic disorder in which enhanced platelet destruction and -weakened platelet production lead to thrombocytopenia. In this study, the p38 mitogen-activated protein kinase (p38-MAPK), early growth response 1 (EGR-1), p53, Bcl-xL, Bak, Bax, and reactive oxygen species (ROS) in platelets from adult patients with chronic ITP were investigated.

METHODS

Platelets were isolated from blood samples collected from 20 adult patients with chronic ITP and 20 healthy volunteers. p38-MAPK, EGR-1, p53, Bcl-xL, Bak, Bax, and ROS were determined by flow cytometry, and the results were analyzed by EXPO32 ADC.

RESULTS

Flow cytometry showed the expression levels of p38-MAPK (61.66 ± 19.38% vs. 27.52 ± 14.34%), EGR-1 (62.22 ± 20.48% vs. 9.05 ± 5.79%), p53 (56.82 ± 20.07% vs. 4.35 ± 2.04%), Bak (39.86 ± 11.45% vs. 20.82 ± 11.85%), Bax (36.85 ± 15.99% vs. 6.69 ± 5.01%), and ROS (19.98 ± 1.47% vs. 1.29 ± 0.10%) were all elevated (p < 0.05 compared with healthy volunteers). In addition, pro-survival Bcl-xL (5.38 ± 1.52% vs. 21.20 ± 6.04%) was decreased markedly in platelets from adult patients with chronic ITP (p < 0.05 compared with healthy volunteers).

CONCLUSIONS

Our findings reveal that platelets in adults with chronic ITP display a proapoptotic gene expression phenotype, based on the enhanced expression of p38-MAPK, EGR-1, p53, Bak, Bax, and ROS, and attenuated expression of Bcl-xL, suggesting increased sensitivity toward apoptosis.

摘要

背景

成人慢性特发性血小板减少性紫癜（ITP）是一种慢性且通常为终身性的出血性疾病，其特征为血小板破坏增强和/或血小板生成减弱导致血小板减少。在这项研究中，我们研究了成人慢性 ITP 患者血小板中的 p38 丝裂原活化蛋白激酶（p38-MAPK）、早期生长反应 1（EGR-1）、p53、Bcl-xL、Bak、Bax 和活性氧（ROS）。

方法

从 20 名成人慢性 ITP 患者和 20 名健康志愿者的血液样本中分离血小板。通过流式细胞术测定 p38-MAPK、EGR-1、p53、Bcl-xL、Bak、Bax 和 ROS，并使用 EXPO32 ADC 进行分析。

结果

流式细胞术显示 p38-MAPK（61.66 ± 19.38% vs. 27.52 ± 14.34%）、EGR-1（62.22 ± 20.48% vs. 9.05 ± 5.79%）、p53（56.82 ± 20.07% vs. 4.35 ± 2.04%）、Bak（39.86 ± 11.45% vs. 20.82 ± 11.85%）、Bax（36.85 ± 15.99% vs. 6.69 ± 5.01%）和 ROS（19.98 ± 1.47% vs. 1.29 ± 0.10%）的表达水平均升高（与健康志愿者相比，p < 0.05）。此外，慢性 ITP 患者的血小板中促生存的 Bcl-xL（5.38 ± 1.52% vs. 21.20 ± 6.04%）显著降低（与健康志愿者相比，p < 0.05）。