Zhou Lijiang, Yang Jun, Ma Siyao, Gao Hong
Department of Oncology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine Shenyang 110032, Liaoning Province, China.
Department of Integrated Chinese and Western Medicine, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute Shenyang 110042, Liaoning Province, China.
Am J Transl Res. 2021 Jul 15;13(7):7723-7729. eCollection 2021.
This study was designed to investigate the effect of micro RNA-targeted regulation of FGFR1 on the proliferation and apoptosis of triple-negative breast cancer (TNBC) cells.
TNBC (MAD-MB-231), three types of breast cancer (MCF10A, MCF7, ZR751) cell lines, and normal breast tissue cell lines were extracted. Real-time PCR was used to detect the expression of miRNA-136 in different types of breast cells. The MAD-MB-231 cell lines were transfected with miRNA-136 mimic by lipofection. The effects of miRNA-136 on FGFR1 expression and apoptosis rate of MAD-MB-231 cell lines were determined using western blotting.
miRNA-136 expression in TNBC cells was lower than that of controls, and was negatively correlated with TNM staging. miRNA-136 expression in MCF10A, MCF7, ZR751, and MAD-MB-231 cell lines was gradually decreased, and MCF10A expression in the other three cell lines was significantly higher than that of MAD-MB-231 cell line (P<0.05). Transfection with miRNA-136 significantly reduced the proliferation rate of MAD-MB-231, and a higher concentration and longer duration exhibited a more pronounced inhibitory effect on proliferation (P<0.05). Transfection with miRNA-136 significantly reduced FGFR1 expression in the MAD-MB-231 cell lines, without significantly affecting apoptosis.
miRNA-136 shows a very low expression level in TNBC cells. Transfection with miRNA-136 can significantly inhibit the proliferation of TNBC cells by external transfection, and has little effect on cell apoptosis. This may be related to miRNA-mediated changes in FGFR1 protein expression.
本研究旨在探讨微小RNA靶向调控FGFR1对三阴性乳腺癌（TNBC）细胞增殖和凋亡的影响。
提取TNBC（MAD-MB-231）、三种乳腺癌（MCF10A、MCF7、ZR751）细胞系以及正常乳腺组织细胞系。采用实时定量聚合酶链反应检测不同类型乳腺细胞中miRNA-136的表达。通过脂质体转染法将miRNA-136模拟物转染至MAD-MB-231细胞系。采用蛋白质免疫印迹法检测miRNA-136对MAD-MB-231细胞系中FGFR1表达和凋亡率的影响。
TNBC细胞中miRNA-136的表达低于对照组，且与TNM分期呈负相关。MCF10A、MCF7、ZR751和MAD-MB-231细胞系中miRNA-136的表达逐渐降低，且MCF10A在其他三种细胞系中的表达显著高于MAD-MB-231细胞系（P<0.05）。转染miRNA-136显著降低了MAD-MB-231的增殖率，且较高浓度和较长时间对增殖的抑制作用更明显（P<0.05）。转染miRNA-136显著降低了MAD-MB-231细胞系中FGFR1的表达，但对凋亡无显著影响。
miRNA-136在TNBC细胞中表达水平极低。通过外部转染miRNA-136可显著抑制TNBC细胞的增殖，对细胞凋亡影响较小。这可能与miRNA介导的FGFR1蛋白表达变化有关。
