Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama, Japan.
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan.
Biosci Biotechnol Biochem. 2021 Sep 22;85(10):2200-2208. doi: 10.1093/bbb/zbab143.
The control of secondary metabolism in fungi is essential for the regulation of various cellular functions. In this study, we searched the RIKEN Natural Products Depository (NPDepo) chemical library for inducers of tenuazonic acid (TeA) production in the rice blast fungus Pyricularia oryzae and identified NPD938. NPD938 transcriptionally induced TeA production. We explored the mode of action of NPD938 and observed that this compound enhanced TeA production via LAE1, a global regulator of fungal secondary metabolism. NPD938 could also induce production of terpendoles and pyridoxatins in Tolypocladium album RK99-F33. Terpendole production was induced transcriptionally. We identified the pyridoxatin biosynthetic gene cluster among transcriptionally induced secondary metabolite biosynthetic gene clusters. Therefore, NPD938 is useful for the control of fungal secondary metabolism.
真菌次生代谢物的控制对于调节各种细胞功能至关重要。在这项研究中，我们从 RIKEN 天然产物库（NPDepo）中筛选了稻瘟病菌（Pyricularia oryzae）中诱导生成雷帕霉素（TeA）的诱导剂，并鉴定出 NPD938。NPD938 转录诱导 TeA 的生成。我们探讨了 NPD938 的作用模式，并观察到该化合物通过真菌次生代谢全局调控因子 LAE1 增强 TeA 的生成。NPD938 还可以诱导 Tolypocladium album RK99-F33 中萜烯醇和吡哆醛的生成。萜烯醇的生成是转录诱导的。我们在转录诱导的次生代谢产物生物合成基因簇中鉴定出了吡哆醛生物合成基因簇。因此，NPD938 可用于控制真菌次生代谢物。
