Singapore Nuclear Research and Safety Initiative, National University of Singapore, Singapore.
Zebrafish. 2021 Oct;18(5):338-341. doi: 10.1089/zeb.2021.0014. Epub 2021 Aug 11.
Cytogenetic approach based on metaphase chromosomes established from dividing cells enables direct microscopic visualization of individual chromosomes, a powerful technique to investigate aneuploidy, chromosome aberrations, and genomic instability. In this study, we describe a simple method based on direct blocking of metaphases in individual zebrafish embryo and dropping slides with temperature changes, water vapor, and acetic acid treatment to increase the metaphase diameters. We demonstrate that well-separated metaphases could be established from single zebrafish embryos using this method. Our method could be further adapted for the analyses of DNA damage, chromosome aberrations, and genomic instability using zebrafish and other teleost models.
基于分裂细胞中期染色体建立的细胞遗传学方法可直接观察单个染色体，这是研究非整倍体、染色体畸变和基因组不稳定性的强大技术。在这项研究中，我们描述了一种简单的方法，该方法基于直接阻断单个斑马鱼胚胎中的中期，然后通过温度变化、水蒸气和乙酸处理来降低玻片的滑动，从而增加中期的直径。我们证明，使用这种方法可以从单个斑马鱼胚胎中建立良好分离的中期。我们的方法可以进一步适应使用斑马鱼和其他硬骨鱼模型进行 DNA 损伤、染色体畸变和基因组不稳定性的分析。
