Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daehak-ro, Yuseong-gu, Daejeon, 34134, Republic of Korea.
Shandong Provincial Key Laboratory of Dryland Farming Technology, Shandong Peanut Industry Collaborative Innovation Center, College of Agronomy, Qingdao Agricultural University, Qingdao, 266000, China.
Theor Appl Genet. 2021 Dec;134(12):3925-3940. doi: 10.1007/s00122-021-03937-5. Epub 2021 Aug 13.
Two major QTL associated with resistance to Fusarium wilt (FW) were identified using whole-genome resequencing. Sequence variations and gene expression level differences suggest that TIR-NBS and LRR-RLK are candidate genes associated with FW-resistance. Fusarium wilt (FW) caused by Fusarium oxysporum f. sp. raphani is an important disease in radish, leading to severe decrease in yield and quality. YR4 as a novel genetic source to resistant to FW was confirmed through screening with five pathogen isolates. We have generated F and F populations segregated with FW resistance using YR4 and YR18 inbred lines. The disease symptom was evaluated in F population (n = 180) in three independent studies over two years. We identified 4 QTL including the two major QTL (FoRsR7.1 and FoRsR9.3). FoRsR7.1 and FoRsR9.3 were detected in three replicated experiments. FoRsR7.1 was delimited to the 2.18-Mb physical interval on chromosome R07, with a high LOD value (5.17-12.84) and explained phenotypic variation (9.34%-27.97%). The FoRsR9.3 represented relatively low LOD value (3.38-4.52) and explained phenotypic variation (6.24%-8.82%). On the basis of the re-sequencing data for the parental lines, we identified five putative resistance-related genes and 13 unknown genes with sequence variations at the gene and protein levels. A semi-quantitative RT-PCR analysis revealed that Rs382940 (TIR-NBS) and Rs382200 (RLK) were expressed only in 'YR4' from 0 to 6 days after the inoculation. Moreover, Rs382950 (TIR-NBS-LRR) was more highly expressed in 'YR4' from 3 to 6 days after the inoculation. These three genes might be important for FW-resistance in radish. We identified several markers based on these potential candidate genes. The marker set should be useful for breeding system to introduce the FW resistance loci from 'YR4' to improve tolerance to FW.
利用全基因组重测序技术鉴定出与枯萎病抗性相关的两个主要 QTL。序列变异和基因表达水平差异表明，TIR-NBS 和 LRR-RLK 是与 FW 抗性相关的候选基因。由尖孢镰刀菌引起的枯萎病（FW）是萝卜的一种重要病害，导致产量和品质严重下降。通过对五个病原菌分离物的筛选，确认 YR4 是一种新型抗 FW 的遗传资源。我们利用 YR4 和 YR18 自交系生成了具有 FW 抗性的 F 和 F 群体。在两年的三个独立研究中，对 F 群体（n=180）的疾病症状进行了评估。我们鉴定出包括两个主要 QTL（FoRsR7.1 和 FoRsR9.3）在内的 4 个 QTL。FoRsR7.1 和 FoRsR9.3 在三个重复实验中均被检测到。FoRsR7.1 被限定在染色体 R07 上的 2.18-Mb 物理区间内，具有较高的 LOD 值（5.17-12.84），解释了表型变异（9.34%-27.97%）。FoRsR9.3 代表相对较低的 LOD 值（3.38-4.52），解释了表型变异（6.24%-8.82%）。基于亲本系的重测序数据，我们在基因和蛋白质水平上鉴定了五个具有序列变异的推定抗相关基因和 13 个未知基因。半定量 RT-PCR 分析显示，Rs382940（TIR-NBS）和 Rs382200（RLK）仅在接种后 0 至 6 天的 'YR4' 中表达。此外，Rs382950（TIR-NBS-LRR）在接种后 3 至 6 天的 'YR4' 中表达更高。这三个基因可能对萝卜的 FW 抗性很重要。我们根据这些潜在候选基因鉴定了几个标记。该标记集应可用于将 'YR4' 中的 FW 抗性基因位点引入到育种系统中，以提高对 FW 的耐受性。
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