College of Food Science and Technology, Ocean University of China, Qingdao 266003, China.
Qingdao Central Medical Group, Qingdao, 266042, China.
Food Funct. 2021 Oct 4;12(19):9087-9097. doi: 10.1039/d1fo00782c.
Antibiotic abuse can lead to gut microbiota disturbance and intestinal inflammation, which in turn may lead to serious inflammatory bowel disease and metabolic syndromes. To investigate the effect of fucoidan on alleviation of the side effects of antibiotics and its structure-activity relationship, we compared the effects of two fucoidan fractions with medium and low molecular weights (MF and LF) from on microbiota dysbiosis, colonic inflammation and intestinal mucosal damage in a cefoperazone-induced intestinal injury mouse model. Our results showed that oral administration of 200 mg kg LF ( = 1.13 kDa) and MF ( = 26.7 kDa) together with 100 mg kg cefoperazone for 10 days could significantly alleviate weight loss, colon shortening and enlargement, mucosal structural damage in the small intestine, cecum and colon induced by cefoperazone in mice. Meanwhile, LF and MF also significantly suppressed the overproduction of TNF-α, IFN-γ, and IL-6 in the colon; however, LF can restore the decrease in the levels of TNF-α and IL-6 in the small intestine and the decrease in the levels of TNF-α, IFN-γ, and IL-6 in the cecum induced by cefoperazone in mice. We found that the molecular weight of fucoidan plays an important role in the regulation of the gut microbiota in antibiotic-treated mice. Interestingly, fucoidans with different molecular weights resulted in quite different caecal microbiota communities. MF exhibited a much better effect on the restoration of the gut microbiota community richness and diversity and the beneficial bacterium . However, LF resulted in the dominance of bacteria including in cefoperazone treated mice, without an increase in the community richness and diversity of caecal microbiota. In the LF and MF treated mice, an increase in the abundance of beneficial bacteria, , and , and a decrease in the abundance of harmful bacteria, , , and were also observed. Considering the negative effect of LF on the gut microbiota, MF with a molecular weight of 26.7 kDa seemed to be a more suitable choice of prebiotics for patients receiving cefoperazone treatment.
抗生素滥用会导致肠道微生物群紊乱和肠道炎症，进而可能导致严重的炎症性肠病和代谢综合征。为了研究褐藻糖胶对缓解抗生素副作用的影响及其结构-活性关系，我们比较了两种来自 的褐藻糖胶中分子质量中等和低的两个馏分（MF 和 LF）对头孢哌酮诱导的肠道损伤小鼠模型中肠道微生物群失调、结肠炎症和肠黏膜损伤的影响。结果表明，连续 10 天口服 200 mg/kg LF（=1.13 kDa）和 MF（=26.7 kDa）与 100 mg/kg 头孢哌酮一起可显著减轻头孢哌酮诱导的小鼠体重减轻、结肠缩短和肿大、小肠、盲肠和结肠黏膜结构损伤。同时，LF 和 MF 还显著抑制了结肠中 TNF-α、IFN-γ 和 IL-6 的过度产生；然而，LF 可以恢复头孢哌酮诱导的小鼠小肠中 TNF-α 和 IL-6 水平的降低以及盲肠中 TNF-α、IFN-γ 和 IL-6 水平的降低。我们发现褐藻糖胶的分子量在调节抗生素处理小鼠的肠道微生物群中起着重要作用。有趣的是，具有不同分子量的褐藻糖胶导致了非常不同的盲肠微生物群落。MF 对恢复肠道微生物群丰富度和多样性以及有益菌 的作用要好得多。然而，LF 导致头孢哌酮处理小鼠中包括 在内的细菌占优势，而盲肠微生物群落的丰富度和多样性没有增加。在 LF 和 MF 处理的小鼠中，还观察到有益菌 的丰度增加， 和 的丰度减少，有害菌 、 、 和 的丰度减少。考虑到 LF 对肠道微生物群的负面影响，分子量为 26.7 kDa 的 MF 似乎是接受头孢哌酮治疗的患者更合适的益生菌选择。
