可注射磷酸钙材料在临界尺寸缺损中的时变反应。

Temporal response of an injectable calcium phosphate material in a critical size defect.

机构信息

Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, 01605, USA.

Graduate School of Biomedical Sciences, University of Massachusetts Medical School, Worcester, MA, 01605, USA.

出版信息

J Orthop Surg Res. 2021 Aug 13;16(1):496. doi: 10.1186/s13018-021-02651-8.

DOI:10.1186/s13018-021-02651-8

PMID:34389027

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8362253/

Abstract

BACKGROUND

Calcium phosphate-based bone graft substitutes are used to facilitate healing in bony defects caused by trauma or created during surgery. Here, we present an injectable calcium phosphate-based bone void filler that has been purposefully formulated with hyaluronic acid to offer a longer working time for ease of injection into bony defects that are difficult to access during minimally invasive surgery.

METHODS

The bone substitute material deliverability and physical properties were characterized, and in vivo response was evaluated in a critical size distal femur defect in skeletally mature rabbits to 26 weeks. The interface with the host bone, implant degradation, and resorption were assessed with time.

RESULTS

The calcium phosphate bone substitute material could be injected as a paste within the working time window of 7-18 min, and then self-cured at body temperature within 10 min. The material reached a maximum ultimate compressive strength of 8.20 ± 0.95 MPa, similar to trabecular bone. The material was found to be biocompatible and osteoconductive in vivo out to 26 weeks, with new bone formation and normal bone architecture observed at 6 weeks, as demonstrated by histological evaluation, microcomputed tomography, and radiographic evaluation.

CONCLUSIONS

These findings show that the material properties and performance are well suited for minimally invasive percutaneous delivery applications.

摘要

背景

磷酸钙基骨移植替代物用于促进创伤或手术引起的骨缺损的愈合。在这里，我们提出了一种可注射的基于磷酸钙的骨空洞填充剂，它与透明质酸一起被专门配制，以提供更长的工作时间，便于将其注入在微创手术中难以进入的骨缺损部位。

方法

对骨替代材料的可输送性和物理性能进行了表征，并在骨骼成熟兔的股骨远端临界尺寸缺损中进行了体内反应评估，时间长达 26 周。随着时间的推移，评估了与宿主骨的界面、植入物降解和吸收。

结果

磷酸钙骨替代材料可以作为糊剂在 7-18 分钟的工作时间窗口内进行注射，然后在 10 分钟内自体温下固化。该材料达到 8.20±0.95MPa 的最大极限抗压强度，类似于小梁骨。该材料在体内被发现具有生物相容性和骨诱导性，直至 26 周，在 6 周时通过组织学评估、微计算机断层扫描和放射学评估观察到新骨形成和正常骨结构。