Department of Gastroenterology, Guizhou Provincial People's Hospital, Guiyang, Guizhou Province, China.
Kaohsiung J Med Sci. 2021 Dec;37(12):1048-1057. doi: 10.1002/kjm2.12437. Epub 2021 Aug 13.
Circular RNAs (circRNAs) feature prominently in regulating the malignant biological behaviors of colorectal cancer (CRC), including cell viability, cell cycle progression, apoptosis, migration, invasion, and so on. This study is performed to probe into the biological function and molecular mechanism of circ_0087862 in CRC. The expression profile of GSE138589 was available from Gene Expression Omnibus (GEO), and the differentially expressed circRNAs were analyzed by GEO2R. The expression of circ_0087862, miR-142-3p, and BACH1 mRNA in CRC tissues and cells was measured by qRT-PCR. CCK-8 assay was employed to determine the proliferation of CRC cells. Scratch wound healing and transwell assays were used to examine the migration and invasion of CRC cells. The targeting relationships between circ_0087862 and miR-142-3p, and between miR-142-3p and BACH1 3'UTR were verified by dual-luciferase reporter gene assay and RIP assay. BACH1 protein expression was probed by western blot. Circ_0087862 was highly expressed in CRC tissues and cell lines. Knocking down circ_0087862 significantly restrained the multiplication, migration and invasion of CRC cells. miR-142-3p inhibition weakened the impact of circ_0087862 knockdown on CRC cells. Circ_0087862 regulated BACH1 expressions by targeting miR-142-3p. Circ_0087862 regulates BACH1 expressions through sponging miR-142-3p, and promotes the proliferation, migration, and invasion of CRC cells.
环状 RNA（circRNAs）在调节结直肠癌（CRC）的恶性生物学行为中起着重要作用，包括细胞活力、细胞周期进程、细胞凋亡、迁移、侵袭等。本研究旨在探讨 circ_0087862 在 CRC 中的生物学功能和分子机制。GEO 数据库中的 GSE138589 数据集可用于分析差异表达的 circRNAs。通过 GEO2R 分析差异表达的 circRNAs。采用 qRT-PCR 检测 CRC 组织和细胞中 circ_0087862、miR-142-3p 和 BACH1 mRNA 的表达。采用 CCK-8 法检测 CRC 细胞的增殖。划痕愈合实验和 Transwell 实验检测 CRC 细胞的迁移和侵袭。通过双荧光素酶报告基因实验和 RIP 实验验证 circ_0087862 与 miR-142-3p 之间、miR-142-3p 与 BACH1 3'UTR 之间的靶向关系。Western blot 检测 BACH1 蛋白表达。circ_0087862 在 CRC 组织和细胞系中高表达。敲低 circ_0087862 显著抑制 CRC 细胞的增殖、迁移和侵袭。抑制 miR-142-3p 减弱了 circ_0087862 敲低对 CRC 细胞的影响。circ_0087862 通过靶向 miR-142-3p 调节 BACH1 的表达。circ_0087862 通过海绵吸附 miR-142-3p 调节 BACH1 的表达，促进 CRC 细胞的增殖、迁移和侵袭。
