Department of Plant Breeding & Genetics, Assam Agricultural University, Jorhat, 785013, Assam, India.
Mol Biotechnol. 2021 Dec;63(12):1280-1292. doi: 10.1007/s12033-021-00380-1. Epub 2021 Aug 16.
Drought is one of the important constraints affecting rice productivity worldwide. The vigorous shoot and deep root system help to improve drought resistance. In present era, genome-wide association study (GWAS) is the preferred method for mapping of QTLs for complex traits such as root and drought tolerance traits. In the present study, 114 rice genotypes were evaluated for various root and shoot traits under water stress conditions. All genotypes showed a significant amount of variation for various root and shoot traits. Correlation analysis revealed that high dry shoot weight and fresh shoot weight is associated with root length, root volume, fresh root weight and dry root weight. A total of 11 significant marker-trait associations were detected for various root, shoot and drought tolerance traits with the coefficient of determination (R) ranging from 18.99 to 53.41%. Marker RM252 and RM212 showed association with three root traits which suggests their scope for improvement of root system. In the present study, a novel QTL was detected for root length associated with RM127, explaining 19.30% of variation. The marker alleles with increasing phenotypic effects for root and drought-tolerant traits can be exploited for improvement of root and drought tolerance traits using marker-assisted selection.
干旱是影响全球水稻生产力的重要限制因素之一。旺盛的 shoot 和 deep root system 有助于提高抗旱性。在当今时代，全基因组关联研究 (GWAS) 是映射复杂性状（如 root 和 drought tolerance 性状）的 QTL 的首选方法。在本研究中，对 114 个水稻基因型在水分胁迫条件下的各种 root 和 shoot 性状进行了评估。所有基因型在各种 root 和 shoot 性状上均表现出显著的变异性。相关性分析表明，高干 shoot 重量和鲜 shoot 重量与 root length、root volume、fresh root weight 和 dry root weight 相关。共检测到 11 个与各种 root、shoot 和耐旱性性状相关的显著 marker-trait 关联，决定系数 (R) 范围为 18.99 至 53.41%。标记 RM252 和 RM212 与三个 root 性状相关，表明它们有改进 root system 的潜力。在本研究中，检测到一个与 RM127 相关的 root length 的新 QTL，解释了 19.30%的变异。具有增加 root 和耐旱性状表型效应的 marker 等位基因可以通过标记辅助选择来改良 root 和耐旱性性状。
