Zhang Wentao, Li Zhen, Du Meiling, Zhang Xiuling, Tian Yaqin, Wang Jinge
College of Food Science Northeast Agricultural University Harbin PR China.
Food Sci Nutr. 2021 Jun 23;9(8):4349-4363. doi: 10.1002/fsn3.2406. eCollection 2021 Aug.
1-MCP is an ethylene inhibitor which can delay the ripening and senescence of fruits and vegetables effectively. (PA) is one of the wild vegetables which is famous and nutrient in China. However, the mechanism of PA preservation treated with 1-MCP has not been reported. Consequently, the effects of postharvest 1-MCP treatment on the changes in quality, energy metabolism, and membrane lipid metabolism of PA were investigated in this study. The results indicated that 1-MCP treatment could effectively inhibit the decreases in firmness, titratable acid (TA) content and the increases in weight loss rate, malondialdehyde (MDA) content, membrane permeability, and membrane lipid metabolism-related enzymes in PA. The cellular energy charge (EC) and the levels of ATP, ATP/ADP, and ATP/AMP, the activities of energy metabolism-related enzymes, NAD, and NADH were maintained, and the decreases in unsaturated fatty acids and the ratio of unsaturated-to-saturated fatty acids in the membrane of PA cells were effectively retarded by 1-MCP treatment. A positive correlation was observed between cellular ATP levels and the ratio of unsaturated-to-saturated fatty acids, while negative correlations were observed between the ratio of unsaturated-to-saturated fatty acids and both lipid peroxidation and membrane permeability. These results indicated that higher levels of energy status, unsaturated-to-saturated fatty acid ratios, and lipid metabolism in the membrane could preserve the membrane integrity of postharvest PA and effectively extend its shelf life.
1-甲基环丙烯（1-MCP）是一种乙烯抑制剂，能有效延缓水果和蔬菜的成熟与衰老。马齿苋（PA）是中国一种著名且营养丰富的野菜。然而，用1-MCP处理PA的保鲜机制尚未见报道。因此，本研究探讨了采后1-MCP处理对PA品质变化、能量代谢和膜脂代谢的影响。结果表明，1-MCP处理能有效抑制PA硬度、可滴定酸（TA）含量的降低以及失重率、丙二醛（MDA）含量、膜透性和膜脂代谢相关酶的增加。维持了细胞能荷（EC）以及ATP、ATP/ADP和ATP/AMP水平、能量代谢相关酶活性、NAD和NADH水平，1-MCP处理有效延缓了PA细胞膜中不饱和脂肪酸及其与饱和脂肪酸比例的降低。细胞ATP水平与不饱和脂肪酸与饱和脂肪酸的比例呈正相关，而不饱和脂肪酸与饱和脂肪酸的比例与脂质过氧化和膜透性均呈负相关。这些结果表明，较高的能量状态、不饱和脂肪酸与饱和脂肪酸比例以及膜中的脂质代谢可以保持采后PA的膜完整性，并有效延长其货架期。
