School of Food Science and Engineering, Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture, Hainan University, Haikou 570228, China.
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China.
Food Chem. 2024 Nov 1;457:140041. doi: 10.1016/j.foodchem.2024.140041. Epub 2024 Jun 13.
Longan fruit deteriorates rapidly after harvest, which limits its storability. This study aimed to investigate the effect of tert-butylhydroquinone (TBHQ) on quality maintenance, membrane lipid metabolism, and energy status of longan fruit during 25 °C storage. Compared with control fruit, TBHQ treatment maintained better marketable fruit rate and suppressed activities of phospholipase D (PLD), lipase, and lipoxygenase (LOX), and downregulated expressions of DlPLD, DlLOX, and Dllipase. TBHQ also increased the ratio of unsaturated fatty acids to saturated fatty acids (U/S) and the index of unsaturated fatty acids (IUFA). In addition, higher levels of ATP, ADP, energy charge, NADP/ NADPH as well as higher activities of H-ATPase, Ca-ATPase and NADK were also observed in TBHQ-treated fruit. These results suggested that TBHQ may maintain postharvest quality of longan fruit by regulating membrane lipid and energy metabolisms.
龙眼果实采后迅速劣变,贮藏期短。本研究旨在探讨叔丁基对苯二酚(TBHQ)对 25℃贮藏期间龙眼果实品质保持、膜脂代谢和能量状态的影响。与对照果实相比,TBHQ 处理能更好地保持可销售果实率,抑制磷脂酶 D(PLD)、脂肪酶和脂氧合酶(LOX)的活性,下调 DlPLD、DlLOX 和 Dllipase 的表达。TBHQ 还增加了不饱和脂肪酸与饱和脂肪酸的比例(U/S)和不饱和脂肪酸指数(IUFA)。此外,TBHQ 处理的果实中还观察到更高水平的 ATP、ADP、能量荷、NADP/NADPH 以及 H-ATPase、Ca-ATPase 和 NADK 的活性。这些结果表明,TBHQ 可能通过调节膜脂和能量代谢来维持龙眼果实的产后品质。