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菠萝蛋白酶对盐水鸭品质的影响。

Effects of bromelain on the quality of smoked salted duck.

作者信息

Ye Ziqing, Zhang Jian, Lorenzo José M, Zhang Mutian, Zhang Wangang

机构信息

Key Lab of Meat Processing and Quality Control Ministry of Education Jiangsu Collaborative Innovation Center of Meat Processing and Quality Control College of Food Science and Technology Nanjing Agricultural University Nanjing China.

Centro Tecnológico de la Carne de Galicia Ourense Spain.

出版信息

Food Sci Nutr. 2021 Jun 24;9(8):4473-4483. doi: 10.1002/fsn3.2422. eCollection 2021 Aug.

Abstract

This study was aimed to assess the effects of bromelain on the eating quality of smoked salted duck. Whole ducks were marinated with different doses of bromelain (300 U/g, 600 U/g, 900 U/g, 1,200 U/g and 1,500 U/g), while the group without bromelain was considered as control (CK). After the production of smoked salted duck was completed, the pH, color, texture, electronic tongue detection, thiobarbituric acid reactive substances (TBARS), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and mass spectrometry analysis were determined. The results showed that, compared to CK, the pH, TBARS and hardness values in 900, 1,200 and 1,500 U/g groups were reduced. The cohesiveness and the springiness were increased while the values of b* were decreased in all bromelain treatments ( < .05). The SDS-PAGE and mass spectrometry analysis indicated that myosin and actin were further hydrolyzed into small-molecule proteins by bromelain. Electronic tongue detection showed that the umami, the saltiness and the richness of smoked salted duck were enhanced, while the bitterness was reduced at the dose of 900 U/g. Thus, bromelain improved the eating quality of smoked salted duck in particular at the level of 900 U/g.

摘要

本研究旨在评估菠萝蛋白酶对盐水鸭食用品质的影响。将整只鸭子用不同剂量的菠萝蛋白酶(300 U/g、600 U/g、900 U/g、1200 U/g和1500 U/g)进行腌制,而未添加菠萝蛋白酶的组作为对照组(CK)。在完成盐水鸭的制作后,测定其pH值、色泽、质地、电子舌检测、硫代巴比妥酸反应物(TBARS)、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和质谱分析。结果表明,与CK组相比,900、1200和1500 U/g组的pH值、TBARS值和硬度值降低。在所有菠萝蛋白酶处理组中,内聚性和弹性增加,而b*值降低(P<0.05)。SDS-PAGE和质谱分析表明,菠萝蛋白酶将肌球蛋白和肌动蛋白进一步水解为小分子蛋白质。电子舌检测表明,盐水鸭的鲜味、咸味和丰富度增强,而在900 U/g剂量下苦味降低。因此,菠萝蛋白酶尤其在900 U/g水平上改善了盐水鸭的食用品质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d28/8358376/e685df0e49e9/FSN3-9-4473-g004.jpg

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