Bitner C, Benjelloun-Touimi S, Dupouey P
Laboratoire de Biochimie des Antigènes, Institut Pasteur, Paris, France.
Brain Res. 1987 Dec 15;465(1-2):167-78. doi: 10.1016/0165-3806(87)90238-0.
The architectural organization of the subpial astrocyte processes was examined near the brain surface by single immunostaining methods. The astroglial processes were stained on brain sections made parallel to the pial surface. The astroglial glial fibrillary acid protein (GFAP) antigen was used as a specific marker. We show that these subpial astrocyte processes present a well organized palisading pattern in the adult mouse and rat spinal cord, medulla and pons. This adult astrocyte palisading pattern is compared to the palisading radial glia organization we previously demonstrated in the fetal mouse brain. The observed analogies afford a new and strong argument in favor of a derivation of the subpial astrocytes from radial glia. Double immunostaining methods, using GFAP and neurofilament antigens as glial and neuronal markers respectively, show the close relationship existing between the trajectories of axonal and glial processes. Beside the colinearity already observed between the axon trajectories and the glial palisades we demonstrate a new kind of axon/glia relationship. Axons are closely intermingled, within the palisading glial tufts, with the peripheral processes of the subpial astrocytes progressing to the pial surface. The findings suggest that fetal radial glia organization has a direct and indirect influence on the adult astroglial and perhaps the axonal pattern.
通过单免疫染色方法，在脑表面附近检查软膜下星形胶质细胞突起的结构组织。在与软膜表面平行制作的脑切片上对星形胶质细胞突起进行染色。使用星形胶质细胞的胶质纤维酸性蛋白（GFAP）抗原作为特异性标记物。我们发现，在成年小鼠和大鼠的脊髓、延髓和脑桥中，这些软膜下星形胶质细胞突起呈现出一种组织良好的栅栏状模式。将这种成年星形胶质细胞的栅栏状模式与我们之前在胎鼠脑中证明的栅栏状放射状胶质细胞组织进行比较。观察到的相似性为软膜下星形胶质细胞源自放射状胶质细胞提供了一个新的有力论据。分别使用GFAP和神经丝抗原作为胶质细胞和神经元标记物的双重免疫染色方法，显示了轴突和胶质细胞突起轨迹之间存在的密切关系。除了已经观察到的轴突轨迹和胶质细胞栅栏之间的共线性之外，我们还证明了一种新的轴突/胶质细胞关系。轴突在栅栏状胶质细胞束内与向软膜表面延伸的软膜下星形胶质细胞的外周突起紧密交织在一起。这些发现表明，胎儿放射状胶质细胞组织对成年星形胶质细胞以及可能对轴突模式有直接和间接的影响。
