Department of Environmental Biochemistry, ICMR-National Institute for Research in Environmental Health, Bhopal, 462030, India.
Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, 226031, India.
Environ Sci Pollut Res Int. 2021 Oct;28(39):54282-54298. doi: 10.1007/s11356-021-15810-5. Epub 2021 Aug 16.
Exposure to polycyclic aromatic hydrocarbons (PAHs) produced from various pyrogenic and petrogenic sources in the environment has been linked to a variety of toxic effects in the human body. Genome-wide analyses have shown that microRNAs (miRNAs) can function as novel and minimally invasive biomarkers of environmental exposure to PAHs. The objective of this study is to explore miRNA signatures associated with early health effects in response to chronic environmental exposure to PAHs. We systematically searched Scopus and PubMed databases for studies related to exposure of PAHs with changes in miRNA expression patterns that represent early health effects in the exposed population. Based on previous studies, we included 15 cell-based and 9 each of animal model and human population-based studies for assessment. A total of 11 differentially expressed PAH-responsive miRNAs were observed each in two or more cell-based studies (miR-181a and miR-30c-1), animal model studies (miR-291a and miR-292), and human population-based studies (miR-126, miR-142-5p, miR-150-5p, miR-24-3p, miR-27a-3p, miR-28-5p, and miR-320b). In addition, miRNAs belonging to family miR-122, miR-199, miR-203, miR-21, miR-26, miR-29, and miR-92 were found to be PAH-responsive in both animal model and cell-based studies; let-7, miR-126, miR-146, miR-30, and miR-320 in both cell-based and human population-based studies; and miR-142, miR-150, and miR-27 were found differentially expressed in both animal model and human population-based studies. The only miRNA whose expression was found to be altered in all the three groups of studies is miR-34c. Association of environmental exposure to PAHs with altered expression of specific miRNAs indicates that selective miRNAs can be used as early warning biomarkers in PAH-exposed population.
暴露于环境中源自各种热解和石油源的多环芳烃（PAHs）已与人体的多种毒性作用相关联。全基因组分析表明，microRNAs（miRNAs）可作为 PAHs 环境暴露的新型微创生物标志物。本研究旨在探索与慢性环境 PAH 暴露相关的早期健康效应相关的 miRNA 特征。我们系统地在 Scopus 和 PubMed 数据库中搜索与 miRNA 表达模式变化相关的 PAHs 暴露研究，这些变化代表了暴露人群的早期健康效应。基于先前的研究，我们纳入了 15 项基于细胞的研究和 9 项动物模型研究和 9 项人群研究进行评估。在两项或更多基于细胞的研究（miR-181a 和 miR-30c-1）、动物模型研究（miR-291a 和 miR-292）和人群研究（miR-126、miR-142-5p、miR-150-5p、miR-24-3p、miR-27a-3p、miR-28-5p 和 miR-320b）中观察到 11 个差异表达的 PAH 反应性 miRNA。此外，miR-122、miR-199、miR-203、miR-21、miR-26、miR-29 和 miR-92 家族的 miRNA 在动物模型和基于细胞的研究中均被发现对 PAH 有反应；在细胞和人群研究中均发现 let-7、miR-126、miR-146、miR-30 和 miR-320 对 PAH 有反应；而 miR-142、miR-150 和 miR-27 在动物模型和人群研究中均表现出差异表达。唯一在所有三组研究中均发现表达改变的 miRNA 是 miR-34c。PAHs 环境暴露与特定 miRNA 表达改变的关联表明，选择性 miRNA 可作为 PAH 暴露人群的早期预警生物标志物。
