Ma Yuxin, Han Jingchao, Luo Xi
Department of Medical Imaging, Ji'nan Stomatologic Hospital, Jinan, Shandong, China.
Department of Stomatology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.
Oral Dis. 2023 Mar;29(2):604-614. doi: 10.1111/odi.14002. Epub 2021 Aug 27.
Recently, increasing attention has been concentrated on decrypting the potential of long non-coding RNAs (lncRNAs) in influencing the progression of human tumors, oral squamous cell carcinoma (OSCC) included. The role of a novel lncRNA, forkhead box D1 antisense RNA 1 (FOXD1-AS1), has been discussed in multiple cancers. Nevertheless, its function and relevant mechanism in OSCC have been not probed yet.
FOXD1-AS1 expression was detected via RT-qPCR. Colony formation, EdU, transwell and Western blot analyses tested the functional role of FOXD1-AS1 in OSCC cells. The relationship between RNAs was assessed by a series of mechanical assays.
FOXD1-AS1 was expressed at a high level in head and neck squamous cell carcinoma (HNSC). Knockdown of FOXD1-AS1 exerted repressive impacts on OSCC cell proliferation, migration, invasion, and EMT. Moreover, FOXD1-AS1 positively regulated its nearby gene FOXD1 via interacting with miR-369-3p. In addition, adenosine deaminase RNA specific (ADAR), known as a RNA-binding protein (RBP), was capable to bind with FOXD1-AS1 and FOXD1 simultaneously, and could regulate the stability of FOXD1 mRNA. Aside from that, rescue assays delineated that FOXD1-AS1 promoted OSCC progression via upregulating FOXD1.
FOXD1-AS1 elevates FOXD1 expression to promote OSCC malignant phenotypes through miR-369-3p and ADAR.
近年来，人们越来越关注解密长链非编码RNA（lncRNA）在影响人类肿瘤进展中的潜力，其中包括口腔鳞状细胞癌（OSCC）。一种新型lncRNA，叉头框D1反义RNA 1（FOXD1-AS1）在多种癌症中的作用已被讨论。然而，其在OSCC中的功能及相关机制尚未得到探究。
通过RT-qPCR检测FOXD1-AS1的表达。采用集落形成、EdU、Transwell和蛋白质免疫印迹分析来测试FOXD1-AS1在OSCC细胞中的功能作用。通过一系列机制分析评估RNA之间的关系。
FOXD1-AS1在头颈部鳞状细胞癌（HNSC）中高表达。敲低FOXD1-AS1对OSCC细胞的增殖、迁移、侵袭和上皮-间质转化具有抑制作用。此外，FOXD1-AS1通过与miR-369-3p相互作用正向调节其附近基因FOXD1。此外，腺苷脱氨酶RNA特异性（ADAR），一种已知的RNA结合蛋白（RBP），能够同时与FOXD1-AS1和FOXD1结合，并可调节FOXD1 mRNA的稳定性。除此之外，挽救实验表明FOXD1-AS1通过上调FOXD1促进OSCC进展。
FOXD1-AS1通过miR-369-3p和ADAR提高FOXD1表达，从而促进OSCC的恶性表型。
