Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea; Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.
Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute for Medical Science, Chungnam National University School of Medicine, Daejeon, Korea.
J Allergy Clin Immunol. 2022 Feb;149(2):599-609.e7. doi: 10.1016/j.jaci.2021.07.037. Epub 2021 Aug 14.
Diverse immune cells contribute to the pathogenesis of chronic rhinosinusitis (CRS), an inflammatory disease of the nasal cavity and paranasal sinuses. However, whether mucosal-associated invariant T (MAIT) cells are present in human sinonasal tissues remains unclear. Furthermore, the characteristics of sinonasal MAIT cells have not been studied in patients with CRS.
We investigated the phenotype, function, and clinical implications of MAIT cells in patients with CRS.
Peripheral blood and sinonasal tissue were obtained from patients with CRS with (CRSwNP) or without nasal polyps (CRSsNP) and healthy controls. MAIT cells were analyzed by flow cytometry.
We found that MAIT cells are present in human sinonasal tissues from healthy controls and patients with CRS. The sinonasal MAIT cell population, but not peripheral blood MAIT cells, from patients with CRSsNP, noneosinophilic CRSwNP (NE-NP), or eosinophilic CRSwNP (E-NP) had a significantly higher frequency of activated cells marked by CD38 expression. In functional analysis, the sinonasal MAIT cell population from NE-NP and E-NP had a significantly higher frequency of IL-17A cells but lower frequency of IFN-γ or TNF cells than control sinonasal tissues. Furthermore, CD38 expression and IL-17A production by sinonasal MAIT cells significantly correlated with disease extent evaluated by the Lund-Mackay computed tomography score in patients with E-NP.
Sinonasal MAIT cells exhibit an activated phenotype and produce higher levels of IL-17A in patients with CRSwNP. These alterations are associated with the extent of disease in patients with E-NP.
多种免疫细胞参与慢性鼻-鼻窦炎（CRS）的发病机制，CRS 是鼻腔和鼻窦的炎症性疾病。然而，鼻黏膜相关不变 T（MAIT）细胞是否存在于人类鼻窦组织中尚不清楚。此外，CRS 患者的鼻黏膜 MAIT 细胞特征尚未得到研究。
我们研究了 MAIT 细胞在 CRS 患者中的表型、功能和临床意义。
从患有 CRS 伴鼻息肉（CRSwNP）或无鼻息肉（CRSsNP）和健康对照者的外周血和鼻窦组织中获得 MAIT 细胞。通过流式细胞术分析 MAIT 细胞。
我们发现 MAIT 细胞存在于健康对照者和 CRS 患者的鼻窦组织中。与外周血 MAIT 细胞相比，CRSsNP、非嗜酸性 CRSwNP（NE-NP）或嗜酸性 CRSwNP（E-NP）患者的鼻窦 MAIT 细胞群具有更高频率的 CD38 表达标记的活化细胞。在功能分析中，NE-NP 和 E-NP 的鼻窦 MAIT 细胞群中，IL-17A 细胞的频率显著更高，而 IFN-γ 或 TNF 细胞的频率显著更低，与 E-NP 患者的 Lund-Mackay 计算机断层扫描评分评估的疾病程度相关。此外，鼻窦 MAIT 细胞的 CD38 表达和 IL-17A 产生与 E-NP 患者的疾病程度显著相关。
CRSwNP 患者的鼻窦 MAIT 细胞表现出活化表型，并产生更高水平的 IL-17A。这些改变与 E-NP 患者的疾病程度有关。
