Tanaka A, Ohtake-Hashiguchi M, Ogawa E
Department of Biology, Faculty of Science, Nara Women's University, Japan.
Growth. 1987 Autumn;51(3):282-300.
Two series of repeated-regeneration experiments were carried out on each fore-, mid- and hindleg of both sexes during the entire postembryonic development period. One was autotomy at trochanterofemoral articulation; the other was amputation from basal coxa. In both experiments, one of the six legs was operated on before the regeneration critical period in the 1st instar. If a regenerate appeared in the following instar, it was repeatedly operated on; if not, operation was postponed until a regenerate appeared. The number of instars required to reach the adult stage increased in both autotomy and amputation experiments. Total larval duration was also prolonged in both experiments. In autotomy regenerates repeatedly appeared almost every instar until the adult stage, while in amputation no regenerates were present just after postoperative molts. Regenerated femur and tibia developed well after autotomy, whereas those after amputation were considerably smaller. All the regenerated legs had tetramerous tarsi. Regenerated legs from autotomized stumps showed incomplete homoeotic regeneration as to the kind of legs. Regenerates from foreleg stumps showed intermediate tibia/femur ratios between normal foreleg and midleg, and those from the hindlegs, intermediate between normal hindleg and midleg. After amputation, the tibia/femur ratios of regenerates were variable. Possible modifiers were suggested for the homeotic and tetramerous regeneration.
在整个胚后发育期间，对两性的前腿、中腿和后腿分别进行了两组重复再生实验。一组是在股骨转子关节处进行自切；另一组是从基节基部进行截肢。在这两个实验中，六条腿中的一条在一龄幼虫的再生关键期之前进行手术。如果在接下来的龄期出现再生肢体，则对其重复进行手术；如果没有出现，则将手术推迟到出现再生肢体时。在自切和截肢实验中，达到成虫阶段所需的龄期数量均增加。两个实验中的幼虫总持续时间也都延长了。在自切实验中，几乎每个龄期都会反复出现再生肢体，直至成虫阶段；而在截肢实验中，术后蜕皮后最初没有再生肢体。自切后再生的股骨和胫骨发育良好，而截肢后的则要小得多。所有再生的腿都有四节跗节。从自切残端再生的腿在腿的类型方面表现出不完全的同源异型再生。从前腿残端再生的腿，其胫股比介于正常前腿和中腿之间；而后腿残端再生的腿，其胫股比介于正常后腿和中腿之间。截肢后，再生腿的胫股比各不相同。针对同源异型和四节再生提出了可能的修饰因子。
