Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, No. 2699, West Gaoke Road, Pudong New District, Shanghai, 201204, China.
Department of Gynecology, Songjiang Maternity and Child Health Hospital, Shanghai, China.
Arch Gynecol Obstet. 2021 Oct;304(4):965-973. doi: 10.1007/s00404-021-06180-x. Epub 2021 Aug 17.
Visualization of the implanted mesh after a pelvic floor repair surgery is important for evaluating mesh-related complications. We made an X-ray-detectable mesh and studied the histocompatibility and toxicity of it.
A thin barium sulfate thread was weaved on a traditional polypropylene mesh to make it X-ray detectable. The cytotoxicity of the mesh was tested by the MTT assay on L929 cell line. The histocompatibility and toxicity of mesh were evaluated in rabbits. Meshes were first implanted intraperitoneally. On postoperative day 7, bloods were tested to estimate the acute toxicity of meshes. After 6 months, rabbits were sacrificed and local inflammatory reaction and tissue regeneration at implantation sites were estimated by the HE stain and Masson stain. In addition, CT scans were performed after surgeries to display the location and shape of implanted meshes.
Compared to the polypropylene mesh group, no significant difference was observed in the X-ray-detectable mesh group on both in vitro cytotoxicity and in vivo acute and chronic toxicity. The amounts of extra cellular matrix between two groups did not differ. Through CT scan and 3D remodeling, the barium sulfate thread clearly revealed the position and shape of the X-ray-detectable mesh, whereas the traditional mesh was invisible under CT scan.
Adding a thin barium sulfate thread on the polypropylene mesh does not change its histocompatibility or toxicity in rabbit model. The barium sulfate thread can effectively show the location and shape of implanted mesh under CT scan.
骨盆底修复手术后，对植入网片进行可视化对于评估与网片相关的并发症非常重要。我们制作了一种可 X 射线检测的网片，并研究了其组织相容性和毒性。
在传统的聚丙烯网片上编织一根细硫酸钡线，使其具有 X 射线可检测性。通过 MTT 法在 L929 细胞系上测试网片的细胞毒性。通过兔模型评估网片的组织相容性和毒性。首先将网片植入腹腔内。术后第 7 天，检测血液以评估网片的急性毒性。6 个月后，处死兔子，通过 HE 染色和 Masson 染色评估植入部位的局部炎症反应和组织再生。此外，在手术后进行 CT 扫描以显示植入网片的位置和形状。
与聚丙烯网片组相比，X 射线可检测网片组在体外细胞毒性以及体内急性和慢性毒性方面均无显著差异。两组之间的细胞外基质量没有差异。通过 CT 扫描和 3D 重建，硫酸钡线清晰地显示了 X 射线可检测网片的位置和形状，而 CT 扫描下传统网片不可见。
在兔模型中，在聚丙烯网上添加细硫酸钡线不会改变其组织相容性或毒性。硫酸钡线可在 CT 扫描下有效显示植入网片的位置和形状。
