Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, China.
College of Life Science, Guangxi Normal University, 15th YuCai St., QiXing District, Guilin, 541004, China.
Environ Sci Pollut Res Int. 2022 Jan;29(3):4101-4115. doi: 10.1007/s11356-021-15948-2. Epub 2021 Aug 17.
In the present study, we characterized the plant growth-promoting traits of Enterobacter sp. FM-1 (FM-1) and investigated its ability to promote growth and increase IAA, P, and Fe concentrations as well as Cd and Pb accumulation in Centella asiatica L. (C. asiatica L.) in upstream area (UA) soil and downstream area (DA) soil that we collected from Siding mine. The results demonstrated that FM-1 secreted IAA, produced siderophores, and had P-solubilization ability even under Cd exposure. IAA secretion reached a maximum of 108.3 ± 1.3 mg L under Cd exposure at 25 mg L. Siderophore production reached a maximum of 0.94 ± 0.01 under Cd exposure at 50 mg L. Pot experiments indicated that FM-1 successfully colonized the roots of C. asiatica L. In both soils, inoculation with FM-1 decreased the pH in rhizosphere soil and increased the bioavailability of both Cd and Pb. In addition, inoculation with FM-1 increased the IAA, P, and Fe concentrations and simultaneously promoted both Cd and Pb accumulation in C. asiatica L. The Cd and Pb concentrations in leaves increased 1.73- and 1.07-fold in the UA soil and 1.25- and 1.11-fold in the DA soil, respectively. Thus, the Cd-resistant strain FM-1 presented excellent PGP traits and could facilitate Cd and Pb phytoremediation by C. asiatica L.
在本研究中，我们对肠杆菌属 FM-1（FM-1）的促植物生长特性进行了描述，并研究了其在来自思丁矿上游区（UA）和下游区（DA）土壤中促进生长、增加吲哚乙酸（IAA）、磷（P）和铁（Fe）浓度以及增加莨菪（Centella asiatica L.）体内 Cd 和 Pb 积累的能力。结果表明，FM-1 在 Cd 暴露下仍能分泌 IAA、产生铁载体并具有 P 溶解能力。在 25 mg L 的 Cd 暴露下，IAA 分泌量最高可达 108.3 ± 1.3 mg L。在 50 mg L 的 Cd 暴露下，铁载体产量最高可达 0.94 ± 0.01。盆栽实验表明，FM-1 成功定植于莨菪的根部。在两种土壤中，接种 FM-1 降低了根际土壤的 pH 值，同时增加了 Cd 和 Pb 的生物有效性。此外，接种 FM-1 增加了 IAA、P 和 Fe 浓度，同时促进了莨菪对 Cd 和 Pb 的积累。在 UA 土壤中，叶片中的 Cd 和 Pb 浓度分别增加了 1.73 倍和 1.07 倍；在 DA 土壤中，叶片中的 Cd 和 Pb 浓度分别增加了 1.25 倍和 1.11 倍。因此，耐 Cd 菌株 FM-1 具有优异的促植物生长特性，可通过莨菪促进 Cd 和 Pb 的植物修复。
