Cocoa Upstream Technology Division, Malaysian Cocoa Board, Sg. Sumun, Malaysia.
Cocoa Downstream Technology Division, Malaysian Cocoa Board, Cocoa Innovative and Technology Centre, Nilai, Malaysia.
J Sci Food Agric. 2022 Mar 15;102(4):1576-1585. doi: 10.1002/jsfa.11494. Epub 2021 Sep 4.
Cacao beans are rich sources of polyphenols with an abundance of flavonoids and methylxanthines that have positive influences on human health. The main factors affecting the formation of flavor as well as the chemical and bioactive composition of cacao beans are cacao pod maturity and post-harvest fermentation. The purpose of this research was to evaluate the effects of pod harvest maturity (mature and ripe) and post-fermentation period (1, 3, and 5 days in a controlled temperature environment) measured by pre-harvest maturity indices, post-harvest quality tests, chemical measurements, and organoleptic evaluation.
As pods developed, flavonol accumulated while nitrogen content degraded. Mature pods produced beans with a higher flavonol, catechin, and total phenolic content (TPC). As fermentation progressed, the beans' fat, TPC, antioxidant activity, and catechin content increased, regardless of pod maturity at harvest. Free fatty acid (FFA) levels were highest in 5 day fermented beans. The 3 day fermented beans contained significantly higher epicatechin, with lower FFA content. Chocolate made from mature beans with 3 day fermentation was more pleasant as it scored the highest in flavor intensity and complexity and the lowest in acidity and astringency.
This study suggests that cacao pods harvested at the mature stage with further fermentation for 3 days under controlled temperatures produce specialty beans with potential health benefits. © 2021 Society of Chemical Industry.
可可豆富含多酚,富含类黄酮和甲基黄嘌呤,对人体健康有积极影响。影响可可豆风味形成以及化学成分和生物活性的主要因素是可可豆荚的成熟度和收获后的发酵。本研究的目的是评估荚果收获成熟度(成熟和完熟)和后发酵期(在受控温度环境中分别为 1、3 和 5 天)对预收获成熟度指数、后收获质量测试、化学测量和感官评价的影响。
随着豆荚的发育,类黄酮积累,而氮含量下降。成熟的豆荚产生的豆具有更高的类黄酮、儿茶素和总酚含量(TPC)。随着发酵的进行,豆子的脂肪、TPC、抗氧化活性和儿茶素含量增加,而与收获时的荚果成熟度无关。游离脂肪酸(FFA)水平在发酵 5 天的豆中最高。发酵 3 天的豆含有更高的表儿茶素,FFA 含量较低。用成熟豆荚和 3 天发酵制成的巧克力味道更宜人,因为它在风味强度和复杂性方面得分最高,而在酸度和涩味方面得分最低。
本研究表明,在受控温度下,成熟豆荚收获并进一步发酵 3 天,可以生产出具有潜在健康益处的特色豆。 © 2021 化学工业协会。