Department of Biology, Tehran Medical Branch, Islamic Azad University, Tehran, Iran.
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
PLoS One. 2021 Aug 18;16(8):e0255920. doi: 10.1371/journal.pone.0255920. eCollection 2021.
Adipokine leptin plays a crucial role in metabolic and reproductive functions. Leptin receptor has a soluble form that binds to leptin, thus modulating its level in the circulation. It has been indicated that the levels of leptin and leptin receptor and also LEP rs7799039 and LEPR rs1137101 polymorphisms are associated with metabolic disorders. In the present study, we assessed the levels of leptin and soluble leptin receptor (sOB-R), and also the frequency of rs7799039 and rs1137101 polymorphisms in healthy fertile women and patients with polycystic ovary syndrome (PCOS), inclusive of PCOS-infertile and PCOS-recurrent pregnancy loss (RPL) subjects.
A total of 324 PCOS patients- including 199 infertile cases and 125 patients with a history of RPL- and 144 healthy controls were enrolled in this study. Biochemical parameters and plasma leptin and sOB-R levels were measured by ELISA and the genotypes of rs7799039 and rs1137101 polymorphisms were determined using PCR- RFLP.
Plasma leptin and sOB-R levels were significantly higher and lower in PCOS, PCOS-infertile and PCOS RPL groups, respectively. The GG genotype frequencies of rs7799039 and rs1137101 polymorphisms were significantly different between PCOS-infertile women and non-PCOS subjects (P = 0.043, OR = 0.47, 95% CI = 0.22-0.97, and P = 0.01, OR = 0.31, 95% CI = 0.12-0.75, respectively). Increased LEP levels were associated with the risk of PCOS and RPL in women with PCOS (P = 0.039, OR = 1.203, 95%CI = [1.009-1.435] and P = 0.012, OR = 1.267, 95% CI = [1.054-1.522], respectively).
Polymorphisms rs7799039 and rs1137101 and circulating leptin and sOB-R levels were associated with infertility in Iranian women with PCOS. Further studies are needed to reveal the role of leptin in PCOS pathogenesis.
脂肪细胞因子瘦素在代谢和生殖功能中起着至关重要的作用。瘦素受体有一个可溶性形式，它与瘦素结合，从而调节其在循环中的水平。已经表明，瘦素和瘦素受体以及 LEP rs7799039 和 LEPR rs1137101 多态性的水平与代谢紊乱有关。在本研究中，我们评估了健康生育妇女和多囊卵巢综合征（PCOS）患者（包括 PCOS 不孕和 PCOS 复发性流产（RPL）患者）的瘦素和可溶性瘦素受体（sOB-R）水平，以及 rs7799039 和 rs1137101 多态性的频率。
本研究共纳入 324 例 PCOS 患者，包括 199 例不孕患者和 125 例有 RPL 病史的患者，以及 144 例健康对照者。通过 ELISA 测定生化参数和血浆瘦素和 sOB-R 水平，通过 PCR-RFLP 确定 rs7799039 和 rs1137101 多态性的基因型。
PCOS、PCOS 不孕和 PCOS RPL 组的血浆瘦素和 sOB-R 水平均显著升高和降低。rs7799039 和 rs1137101 多态性的 GG 基因型频率在 PCOS 不孕妇女和非 PCOS 患者之间存在显著差异（P=0.043，OR=0.47，95%CI=0.22-0.97，P=0.01，OR=0.31，95%CI=0.12-0.75）。在 PCOS 患者中，LEP 水平升高与 PCOS 和 RPL 的风险相关（P=0.039，OR=1.203，95%CI=[1.009-1.435]，P=0.012，OR=1.267，95%CI=[1.054-1.522]）。
伊朗 PCOS 不孕妇女的 rs7799039 和 rs1137101 多态性、循环瘦素和 sOB-R 水平与不孕有关。需要进一步研究以揭示瘦素在 PCOS 发病机制中的作用。
