皮考汀醇对恶性黑素瘤的作用及其机制
Effect of piceatannol against malignant melanoma and .
机构信息
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2021 Aug 1;39(4):413-418. doi: 10.7518/hxkq.2021.04.006.
OBJECTIVES
To study the antitumor effect of piceatannol (PIC) on malignant melanoma and .
METHODS
B16F10 cells were cultured and treated with gradient concentrations of PIC. Cell viability was detected with methyl thiazolyl tetrazolium (MTT) assay; matrix metalloproteinase (MMP)-2, MMP-9, vascular endothelial growth factor (VEGF), spleen tyrosine kinase (Syk), and p-Syk were detected with Western blot; migration ability was detected with wound healing assay; invasion ability was detected with Transwell assay. Syk expression was suppressed through RNA interference for the detection of the possible mechanism of PIC in melanoma. An study was established by creating B16F10-bearing mice with intraperitoneal injection of PIC.
RESULTS
The cell viability of B16F10 decreased with increasing PIC concentration. The results of the Transwell assay showed that invasion ability decreased with increasing PIC concentration, and healing time was prolonged at increased PIC concentration in the wound healing assay. Western blot results showed that PIC mainly inhibited the phosphorylation of Syk and inhibited the expression of MMP-2, MMP-9, and VEGF. RNA interference pointed out that blocking the expression of Syk can reveal the same inhibition effect on B16F10 cells as PIC. study revealed that different concentrations of PIC cangreatly inhibit melanoma progression.
CONCLUSIONS
PIC might block the progression of malignant melanoma by inhibiting spleen tyrosine kinase.
目的
研究白藜芦醇（PIC）对恶性黑色素瘤的抗肿瘤作用。
方法
培养 B16F10 细胞，并使用梯度浓度的 PIC 进行处理。通过噻唑蓝（MTT）法检测细胞活力；通过 Western blot 检测基质金属蛋白酶（MMP）-2、MMP-9、血管内皮生长因子（VEGF）、脾酪氨酸激酶（Syk）和磷酸化 Syk（p-Syk）的表达；通过划痕愈合实验检测迁移能力；通过 Transwell 实验检测侵袭能力。通过 RNA 干扰抑制 Syk 表达，以检测 PIC 对黑色素瘤的可能作用机制。通过腹腔注射 PIC 建立 B16F10 荷瘤小鼠模型进行研究。
结果
B16F10 细胞的细胞活力随 PIC 浓度的增加而降低。Transwell 实验结果表明，侵袭能力随 PIC 浓度的增加而降低，划痕愈合实验中 PIC 浓度增加时愈合时间延长。Western blot 结果表明，PIC 主要抑制 Syk 的磷酸化，并抑制 MMP-2、MMP-9 和 VEGF 的表达。RNA 干扰表明，阻断 Syk 的表达可以揭示 PIC 对 B16F10 细胞的相同抑制作用。体内研究表明，不同浓度的 PIC 可以极大地抑制黑色素瘤的进展。
结论
PIC 可能通过抑制脾酪氨酸激酶来阻止恶性黑色素瘤的进展。
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