Neonatal Research Group, Health Research Institute Hospital La Fe, Valencia, Spain.
Division of Radiology and Imaging, University and Polytechnic Hospital La Fe, Valencia, Spain.
Antioxid Redox Signal. 2021 Dec 10;35(17):1467-1475. doi: 10.1089/ars.2021.0168.
The pathogenesis and progression of hypoxic-ischemic encephalopathy (HIE), a major cause of severe neurological disability and mortality in the perinatal period, are shaped by the interplay of multiple processes, including inflammation, oxidative stress, and excitotoxicity. We conducted a longitudinal study to determine biomarkers of oxidative stress and inflammation in noninvasive urine samples of newborns with moderate/severe HIE ( = 51), employing liquid chromatography-mass spectrometry. We noted that levels of several biomarkers of oxidative stress increased over time, demonstrating the ongoing propagation of oxidative injury. Prostaglandins, in contrast, showed a decreasing trend in their concentration profiles over time, which probably reflects their mediation in pathogenic mechanisms, including the inflammatory response. Statistically significant differences in the levels of oxidative stress of neonates with distinct brain lesion patterns, as detected with magnetic resonance imaging (MRI), were observed, revealing an increase of lipid peroxidation biomarkers in newborns with cerebral lesions (MRI score of 1 compared with scores of 0 and 2). Moreover, a gender-dependent study showed no statistically significant differences in biomarker concentrations between male and female infants. Our observation leads to the hypothesis that monitoring of noninvasive lipid peroxidation biomarkers could aid in diagnosis and prediction of long-term outcomes as a complementary tool to standard exploration. 35, 1467-1475.
缺氧缺血性脑病（HIE）是围产期严重神经功能障碍和死亡的主要原因，其发病机制和进展受多种过程的相互作用影响，包括炎症、氧化应激和兴奋性毒性。我们进行了一项纵向研究，使用液相色谱-质谱法，在患有中重度 HIE 的新生儿的非侵入性尿液样本中确定氧化应激和炎症的生物标志物。我们注意到，几种氧化应激生物标志物的水平随时间推移而增加，表明氧化损伤持续发展。相比之下，前列腺素的浓度曲线随时间呈下降趋势，这可能反映了它们在包括炎症反应在内的发病机制中的介导作用。通过磁共振成像（MRI）检测到具有不同脑损伤模式的新生儿之间的氧化应激水平存在统计学显著差异，显示出脑损伤新生儿（MRI 评分 1 与 0 和 2 相比）中脂质过氧化生物标志物的增加。此外，一项性别依赖性研究表明，男婴和女婴之间的生物标志物浓度没有统计学显著差异。我们的观察结果提出了这样一种假设，即监测非侵入性脂质过氧化生物标志物可以作为标准探索的补充工具，有助于诊断和预测长期结局。35, 1467-1475。
