College of Animal Medicine, Jilin Agricultural University, Changchun 130118, P.R. China.
College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, P.R. China.
J Microbiol Biotechnol. 2021 Oct 28;31(10):1350-1357. doi: 10.4014/jmb.2105.05013.
() is a major pathogen that causes human pneumonia, leading to significant morbidity and mortality. coagulase (Coa) triggers the polymerization of fibrin by activating host prothrombin, which then converts fibrinogen to fibrin and contributes to pathogenesis and persistent infection. In our research, we demonstrate that isovitexin, an active traditional Chinese medicine component, can inhibit the coagulase activity of Coa but does not interfere with the growth of . Furthermore, we show through thermal shift and fluorescence quenching assays that isovitexin directly binds to Coa. Dynamic simulation and structure-activity relationship analyses suggest that V191 and P268 are key amino acid residues responsible for the binding of isovitexin to Coa. Taken together, these data indicate that isovitexin is a direct Coa inhibitor and a promising candidate for drug development against infection.
（）是一种主要的病原体，可引起人类肺炎，导致发病率和死亡率显著增加。凝固酶（Coa）通过激活宿主凝血酶原来触发纤维蛋白的聚合，然后将纤维蛋白原转化为纤维蛋白，导致发病机制和持续感染。在我们的研究中，我们证明了异牡荆素，一种活性的中药成分，能够抑制 Coa 的凝固酶活性，但不干扰的生长。此外，我们通过热迁移和荧光猝灭实验表明，异牡荆素直接与 Coa 结合。动态模拟和构效关系分析表明，V191 和 P268 是异牡荆素与 Coa 结合的关键氨基酸残基。总之，这些数据表明，异牡荆素是 Coa 的直接抑制剂，是针对感染开发药物的有前途的候选物。
