超声辐射对表皮葡萄球菌生物膜的抑制作用。
Inhibitory effects of ultrasound irradiation on Staphylococcus epidermidis biofilm.
机构信息
Department of Clinical Laboratory Medicine, Jichi Medical University, 3311-1 Yakushiji Shimotsuke-Shi, Tochigi, 329-0498, Japan.
Department of Human Health Science, Kyoto University Graduate School of Medicine, Kyoto, Japan.
出版信息
J Med Ultrason (2001). 2021 Oct;48(4):439-448. doi: 10.1007/s10396-021-01120-3. Epub 2021 Aug 19.
PURPOSE
We aimed to investigate whether low-intensity continuous and pulsed wave ultrasound (US) irradiation can inhibit the formation of Staphylococcus epidermidis biofilms, for potential application in the treatment of catheter-related bloodstream infections (CRBSI).
METHODS
S. epidermidis biofilms that formed on the bottom surfaces of 6-well plates were irradiated on the bottom surface using the sound cell incubator system for different intervals of time.
RESULTS
US irradiation with continuous waves for 24 h notably inhibited biofilm formation (p < 0.01), but the same US irradiation for 12 h had no remarkable effect. Further, double US irradiation with pulsed waves for 20 min inhibited biofilm formation by 33.6%, nearly two-fold more than single US irradiation, which reduced it by 17.9%.
CONCLUSION
US irradiation of a lower intensity (I = 6-29 mW/cm) than used in a previous study and lower than recommended by the Food and Drug Administration shows potential for preventing CRBSI caused by bacterial biofilms.
目的
我们旨在研究低强度连续波和脉冲波超声（US）辐射是否能抑制表皮葡萄球菌生物膜的形成，以期应用于治疗导管相关血流感染（CRBSI）。
方法
采用声细胞孵育系统对 6 孔板底部表面形成的表皮葡萄球菌生物膜进行不同时间间隔的底部表面辐照。
结果
连续波 24 h 的 US 辐射显著抑制生物膜形成（p<0.01），但相同的 12 h US 辐射则无明显效果。此外，20 min 的双脉冲波 US 辐射抑制生物膜形成的效果为 33.6%，几乎是单次 US 辐射（抑制 17.9%）的两倍。
结论
与之前研究中使用的强度（I=6-29 mW/cm）以及低于食品和药物管理局推荐强度相比，较低强度的 US 辐射显示出预防由细菌生物膜引起的 CRBSI 的潜力。
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