Chen Xiang-Ying, Li Meng-Wei, Wang Ying, Chen Quan, Xu Han-Mei
Engineering Research Center of Synthetic Peptide Drug Discovery and Evaluation of Jiangsu Province, China Pharmaceutical University, Nanjing 211198, China.
Yi Chuan. 2021 Aug 20;43(8):737-746. doi: 10.16288/j.yczz.21-167.
Existing research has shown that there are a large amount of non-coding RNAs (ncRNAs) in organisms. Short open reading frames (sORFs) abundantly exist in molecular sequences inaccurately annotated as ncRNAs. Several sORFs can be transcribed and translated into evolutionarily conserved micropeptides, which were ignored in previous studies due to short sequence lengths and the limitations of research techniques. To date, sORF-encoded micropeptides with various functions have been found to play important roles in regulating vital biological activities. This article reviews the functional micropeptides which have been found in recent years, introduces the new micropeptide designated as MIAC that we have discovered and describes the related technologies for mining potential micropeptides, thereby providing insights and references for new micropeptide discovery for researchers.
现有研究表明，生物体内存在大量非编码RNA（ncRNA）。短开放阅读框（sORF）大量存在于被错误注释为ncRNA的分子序列中。一些sORF可以被转录并翻译成具有进化保守性的小肽，由于序列长度较短和研究技术的局限性，这些小肽在以往的研究中被忽视了。迄今为止，已发现具有各种功能的sORF编码小肽在调节重要生物活性中发挥重要作用。本文综述了近年来发现的功能性小肽，介绍了我们发现的名为MIAC的新小肽，并描述了挖掘潜在小肽的相关技术，从而为研究人员发现新的小肽提供思路和参考。
