Fruns M, Krieger C, Sears T A
Sobell Department of Neurophysiology, Institute of Neurology, London, U.K.
Neurosci Lett. 1987 Dec 16;83(1-2):82-8. doi: 10.1016/0304-3940(87)90220-5.
The retrograde transport of fluorochromes from muscle was used to label embryonic rat motoneurones in vivo. The fluorescent motoneurones were subsequently detected in cultures of dissociated spinal cord neurones using an image-intensification video camera, the images from which allowed identification of the motoneurones under phase-contrast microscopy. Such motoneurones were mostly phase dark, often with a multipolar appearance and were detectable for up to 4 days in culture. Whole-cell patch-clamp recordings from these motoneurones demonstrated mean input resistances of 635 M omega and resting membrane potentials of -65 mV; action potentials could be evoked and, under voltage-clamp, inward and outward currents were present.
利用荧光染料从肌肉的逆行运输在体内标记胚胎大鼠运动神经元。随后，使用图像增强摄像机在解离的脊髓神经元培养物中检测到荧光运动神经元，其图像可在相差显微镜下识别运动神经元。此类运动神经元大多呈暗相，常呈多极外观，在培养中最多可检测4天。对这些运动神经元进行的全细胞膜片钳记录显示，平均输入电阻为635 MΩ，静息膜电位为-65 mV；可诱发动作电位，在电压钳下，存在内向和外向电流。
