Kataoka S, Yamamoto T Y, Yui R
Department of Anatomy, Tohoku University School of Medicine, Sendai.
Tohoku J Exp Med. 1987 Dec;153(4):335-46. doi: 10.1620/tjem.153.335.
Serotonin (5-HT)-immunohistochemistries at light- and electron-microscopic levels, using rabbit anti-5-HT serum (#1234), were applied to the whole head and only to the eye of the abalone, respectively. Peroxidase-antiperoxidase and fluorescein isothiocyanate methods were used for the light-microscopic immunohistochemistries. Many immunoreactive nerve fibers were demonstrated in the outer zone of the retinal plexiform layer, small optic nerve fiber bundles, the optic nerve trunk and the cerebral ganglion. Immunoreactive somata were observed only in the cerebral ganglion. Accordingly the immunoreactive fibers in the retinal plexiform layer are considered to be efferent. Cored vesicles in the retinal plexiform layer demonstrated by both conventional chemofixation and a rapid-freeze-substitution method showed strong immunoreactivities localized within their limiting membrane. The same fibers also contained small clear vesicles. They are considered to be different from larger clear vesicles in non-immunoreactive fibers reportedly containing acetylcholine. The function of the efferent fibers remains to be elucidated.
使用兔抗5-羟色胺血清（#1234），分别在光镜和电镜水平对鲍鱼的整个头部和仅对其眼睛进行了5-羟色胺（5-HT）免疫组织化学分析。光镜免疫组织化学采用过氧化物酶-抗过氧化物酶法和异硫氰酸荧光素法。在视网膜丛状层的外层区域、小的视神经纤维束、视神经干和脑神经节中发现了许多免疫反应性神经纤维。仅在脑神经节中观察到免疫反应性胞体。因此，视网膜丛状层中的免疫反应性纤维被认为是传出纤维。通过传统化学固定和快速冷冻置换法在视网膜丛状层中显示的有芯小泡表现出强烈的免疫反应性，定位在其界膜内。同样的纤维还含有小的清亮小泡。据报道，它们被认为与含有乙酰胆碱的非免疫反应性纤维中的较大清亮小泡不同。传出纤维的功能仍有待阐明。
