[Simulation of the neurotrophic control of the resting membrane potential in rat skeletal muscle by using electrical stimulation and an exogenous cholinomimetic].

Decrease of the resting MP caused by denervation of the rat diaphragm muscle, was studied in vitro. Addition of carbamylcholine as well as electrical stimulation hyperpolarized the muscle membrane, the effect of the former being not prevented by d-tubocurarine. The hyperpolarizing effects of carbamylcholine and electrical stimulation used simultaneously were not added up. The resting MP in muscles with long stump of the nerve was not affected by d-tubocurarine added to the culturing medium. The action of synaptic acetylcholine via d-tubocurarine-insensitive mechanism and the nerve impulsation seem to serve as additional factors in complete actualization of the resting MP neurotrophic control.