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用于多细胞系统中顺磁性离子转运研究的核磁共振技术。

1H NMR techniques in studies of transport of paramagnetic ions in multicellular systems.

作者信息

Ratković S, Bacić G

机构信息

Department of Technology and Chemical Research, Maiz Research Institute Zemun Polje, Zemun-Beograd, Yugoslavia.

出版信息

Gen Physiol Biophys. 1987 Dec;6(6):609-15.

PMID:3443285
Abstract

Two different pulse sequences used in 1H NMR spectroscopy termed free induction decay amplitude recovery (FIDAR) and spin-echo recovery (SER) were applied to studies of transport of paramagnetic ions in multicellular systems. The molar relaxivity of several paramagnetic species (Fe3+, Co2+, Ni2+, Cu2+, Mn2+, MnEDTA2-, dextran-magnetite) in water solutions was measured at 32 MHz resonance frequency. Ionic transport was studied using Mn2+ and MnEDTA2- as models for cations and anions, respectively, and plant root tissue as a model of a multicellular system.

摘要

在1H核磁共振光谱学中使用的两种不同脉冲序列,即自由感应衰减幅度恢复(FIDAR)和自旋回波恢复(SER),被应用于多细胞系统中顺磁性离子传输的研究。在32兆赫共振频率下测量了几种顺磁性物质(Fe3+、Co2+、Ni2+、Cu2+、Mn2+、MnEDTA2-、葡聚糖-磁铁矿)在水溶液中的摩尔弛豫率。分别使用Mn2+和MnEDTA2-作为阳离子和阴离子的模型,以及植物根组织作为多细胞系统的模型来研究离子传输。

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