在顺磁中心存在的情况下，通过质子核磁共振研究植物组织中的水分交换。
Water exchange in plant tissue studied by proton NMR in the presence of paramagnetic centers.
作者信息
Bacić G, Ratković S
出版信息
Biophys J. 1984 Apr;45(4):767-76. doi: 10.1016/S0006-3495(84)84220-4.
The proton NMR relaxation of water in maize roots in the presence of paramagnetic centers, Mn2+, Mn- EDTA2 -, and dextran-magnetite was measured. It was shown that the NMR method of Conlon and Outhred (1972, Biochem. Biophys. Acta. 288:354-361) can be applied to a heterogenous multicellular system, and the water exchange time between cortical cells and the extracellular space can be calculated. The water exchange is presumably controlled by the intracellular unstirred layers. The Mn- EDTA2 - complex is a suitable paramagnetic compound for complex tissue, while the application of dextran-magnetite is probably restricted to studies of water exchange in cell suspensions. The water free space of the root and viscosity of the cells cytoplasm was estimated with the use of Mn- EDTA2 -. The convenience of proton NMR for studying the multiphase uptake of paramagnetic ions by plant root as well as their transport to leaves is demonstrated. A simple and rapid NMR technique (spin-echo recovery) for continuous measurement of the uptake process is presented.
测定了在顺磁中心、Mn2+、Mn-EDTA2-和葡聚糖-磁铁矿存在下玉米根中水分的质子核磁共振弛豫。结果表明，Conlon和Outhred（1972年，《生物化学与生物物理学报》288:354 - 361）的核磁共振方法可应用于异质多细胞系统，并且可以计算皮层细胞与细胞外空间之间的水分交换时间。水分交换大概受细胞内未搅拌层控制。Mn-EDTA2-复合物是用于复杂组织的合适顺磁化合物，而葡聚糖-磁铁矿的应用可能仅限于细胞悬浮液中水分交换的研究。利用Mn-EDTA2-估计了根的自由水空间和细胞细胞质的粘度。证明了质子核磁共振在研究植物根系对顺磁离子的多相吸收及其向叶片运输方面的便利性。提出了一种用于连续测量吸收过程的简单快速的核磁共振技术（自旋回波恢复）。
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