Zhou Yongjian, Huang Nanqu, Li Yuanyuan, Ba Zhisheng, Zhou Yanjun, Luo Yong
Department of Neurology, Xiangtan Central Hospital, Xiangtan, Hunan, China.
Department of Neurology, Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi), Zunyi, Guizhou, China.
PeerJ. 2021 Aug 10;9:e11978. doi: 10.7717/peerj.11978. eCollection 2021.
The aim of this study was to investigate the effect of icaritin (ICT) on TAR DNA-binding protein 43 (TDP-43)-induced neuroblastoma (SH-SY5Y) cell damage and to further explore its underlying mechanisms.
To investigate the possible mechanism, TDP-43 was used to induce SH-SY5Y cell injury. Cell viability was evaluated by the CCK-8 assay. The mitochondrial membrane potential (MMP) was determined with JC-1. The expression levels of TDP-43 and cytochrome C (CytC) were measuring by Western blotting. Changes in adenosine 5'-triphosphate (ATP) content, total antioxidative capacity (T-AOC), glutathione peroxidase (GSH-Px) activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were detected with specific kits.
The results showed that ICT reduced the cell damage induced by TDP-43. ICT reduced the expression level of TDP-43; increased ATP content and the MMP; decreased CytC expression; increased T-AOC and GSH-Px, total SOD (T-SOD), copper/zinc SOD (CuZn-SOD), and manganese SOD (Mn-SOD) activity; and decreased MDA content.
The results suggest that ICT has a protective effect on TDP-43-transfected SH-SY5Y cells that is related to reductions in TDP-43 expression and mitochondrial damage and alleviation of oxidative stress.
本研究旨在探讨淫羊藿素（ICT）对TAR DNA结合蛋白43（TDP-43）诱导的神经母细胞瘤（SH-SY5Y）细胞损伤的影响，并进一步探究其潜在机制。
为探究可能的机制，采用TDP-43诱导SH-SY5Y细胞损伤。通过CCK-8法评估细胞活力。用JC-1检测线粒体膜电位（MMP）。通过蛋白质免疫印迹法检测TDP-43和细胞色素C（CytC）的表达水平。使用特定试剂盒检测三磷酸腺苷（ATP）含量、总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）活性、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量的变化。
结果显示，ICT减轻了TDP-43诱导的细胞损伤。ICT降低了TDP-43的表达水平；增加了ATP含量和MMP；降低了CytC表达；增加了T-AOC和GSH-Px、总SOD（T-SOD）、铜/锌SOD（CuZn-SOD）和锰SOD（Mn-SOD）活性；并降低了MDA含量。
结果表明，ICT对TDP-43转染的SH-SY5Y细胞具有保护作用，这与TDP-43表达降低、线粒体损伤减轻和氧化应激缓解有关。
