Department of Basic Sciences, Faculty of veterinary Medicine, University of Tehran, Tehran, Iran.
Core Centre for Molecular Morphology, Section for Stereology and Microscopy, Aarhus University; Department of Pathology, Aarhus University Hospital, Aarhus, Denmark.
Microsc Res Tech. 2021 Dec;84(12):2915-2921. doi: 10.1002/jemt.23849. Epub 2021 Aug 26.
The development of metanephros is a complex and gradual process. The number, size and distribution of nephrons provide important information about the organization of the kidney. Stereology is the current gold-standard technique for the morphometrical evaluation of kidney structures. This study describes morphometric features of the kidney development in sheep using design-based stereological techniques aimed to introduce the sheep as a translational model in human nephrogenesis. Left kidneys of 16 sheep fetuses in four groups at 9-11, 12-14, 15-17, and 18-20 weeks of gestation were used in the present study. Systematic uniform random sections were obtained. The kidney volume, volume fraction of nephrogenic zone, cortex and medulla, and glomerular volume were estimated using point counting and Cavalieri's estimator. The total glomerular number was estimated using a physical disector/fractionator technique. The kidney and its compartments presented gradual changes with aging, with differences found in the last fetal ages. The kidney volume increased from 0.94 ± 0.22 cm to 8.6 ± 0.88 cm during development. The volume of cortex increased from 406 ± 85 mm to 5,151 ± 309 mm and the volume of medulla showed increase from 301 ± 91 mm to 3,426 ± 599 mm . The total glomerular volume increased from 13.8 ± 1.6 mm to 235 ± 44 mm . The total glomerular number increased from 4,683 ± 757 to 639 × 10 ± 11.6 × 10 . Our data might contribute to the knowledge of embryological urology and promote future experimental investigations in this field.
后肾的发育是一个复杂而渐进的过程。肾单位的数量、大小和分布为肾脏的组织结构提供了重要信息。体视学是目前评估肾脏结构形态计量学的金标准技术。本研究使用基于设计的体视学技术描述了绵羊肾脏发育的形态学特征，旨在将绵羊作为人类肾发生的转化模型引入。本研究使用了 16 只绵羊胎儿的左肾，这些胎儿分为 4 组，分别在妊娠 9-11、12-14、15-17 和 18-20 周龄。采用系统随机均匀取样方法获取样本。使用点计数和 Cavalieri 估计法估算了肾体积、肾发生区、皮质和髓质的体积分数以及肾小球体积。使用物理割除法/分馏器技术估计了总肾小球数。随着胎儿的发育，肾脏及其各部分逐渐发生变化，在最后几个胎儿期发现了差异。肾脏体积从 0.94±0.22cm增加到 8.6±0.88cm。皮质体积从 406±85mm增加到 5151±309mm，髓质体积从 301±91mm增加到 3426±599mm。总肾小球体积从 13.8±1.6mm增加到 235±44mm。总肾小球数从 4683±757 增加到 639×10±11.6×10。我们的数据可能有助于胚胎泌尿学的知识，并促进该领域未来的实验研究。
