Poznan Research Centre, Department of Genetics and Breeding of Oilseed Crop, Plant Breeding and Acclimatization Institute-National Research Institute, 60-479 Poznań, Poland.
School of Biological Sciences, University of Western Australia, Crawley, WA 6009, Australia.
Genes (Basel). 2021 Aug 11;12(8):1235. doi: 10.3390/genes12081235.
Rapeseed ( L.) meal is an important source of protein, but the presence of anti-nutritional compounds, such as fibre and glucosinolates, still limits its use as a livestock feed. Understanding the genetic basis of seed fibre biosynthesis would help to manipulate its content in seeds of oilseed rape. Here, we applied high-resolution skim genotyping by sequencing (SkimGBS) and characterised 187,835 single-nucleotide polymorphism (SNP) markers across a mapping population subsequently used for a genetic mapping study (R/qtl). This approach allowed the identification of 11 stable QTL related to seed quality traits and led to the identification of potential functional genes underlying these traits. Among these, key genes with a known role in carbohydrate metabolic process, cell wall, lignin, and flavonoid biosynthesis, including cellulase , , , and , were found. This study furthers the understanding of the molecular mechanisms underlying seed fibre content and provides new markers for molecular breeding in .
油菜籽粕是一种重要的蛋白质来源，但由于存在抗营养化合物，如纤维和硫代葡萄糖苷，其作为牲畜饲料的应用仍然受到限制。了解种子纤维生物合成的遗传基础将有助于控制油菜籽中纤维的含量。在这里，我们应用了高分辨率脱脂基因组测序（SkimGBS）技术，并在随后用于遗传图谱研究（R/qtl）的作图群体中对 187835 个单核苷酸多态性（SNP）标记进行了特征描述。这种方法可以识别与种子质量性状相关的 11 个稳定的 QTL，并鉴定出这些性状潜在的功能基因。其中，包括纤维素酶在内的与碳水化合物代谢过程、细胞壁、木质素和类黄酮生物合成有关的关键基因，都被发现了。这项研究进一步了解了种子纤维含量的分子机制，并为油菜的分子育种提供了新的标记。
