Department of Orthopedics, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, PR China.
Department of General Surgery, Chinese Academy of Medical Sciences, Peking Union Medical College, Peking Union Medical College Hospital, Beijing 100730, PR China.
J Biomed Nanotechnol. 2021 Jul 1;17(7):1330-1338. doi: 10.1166/jbn.2021.3110.
The best way in which to prepare scaffolds with good biological properties is an urgent problem in the field of tissue engineering. In this paper we discuss the preparation of nano-hydroxyapatite scaffold of recombinant human bone morphogenetic protein-2 (rhBMP-2) and its application in bone defect repair. rhBMP-2 reagent was dissolved in 1 mol/L sodium dihydrogen phosphate solution, and the rhBMP-2 solution was added to the nano-hydroxyapatite artificial bone with a 100 L glass micro dropper at the rate of 10 drops/min to obtain Nano-HA/rhBMP-2 composite artificial bone. In experiments, rabbits were fixed on an operating table, a 2 cm longitudinal incision was made in the middle part of the radial forearm, and the radius was cut with a wire saw and periosteum, 2.5 cm away from the distal radius. After washing the wound with normal saline, Adv-hBMP-2/MC3T3-E1 nano-HA composite artificial bone, MC3T3-E1 nan-HA composite artificial bone, or Nano-HA artificial bone were implanted in different groups. The artificial bone scaffold prepared in this study has a stronger ability to repair bone defects than the alternatives, and is a promising prospect for the clinical treatment of bone defects.
制备具有良好生物性能的支架的最佳方法是组织工程领域的一个迫切问题。本文探讨了重组人骨形态发生蛋白-2（rhBMP-2）纳米羟基磷灰石支架的制备及其在骨缺损修复中的应用。rhBMP-2 试剂溶解于 1mol/L 磷酸二氢钠溶液中，以 10 滴/分钟的速度用 100μL 玻璃微量移液器将 rhBMP-2 溶液滴加到纳米羟基磷灰石人工骨上，得到 Nano-HA/rhBMP-2 复合材料人工骨。在实验中，将兔子固定在手术台上，在前臂桡骨中部做 2cm 长的纵切口，用钢丝锯和骨膜切开桡骨，距桡骨远端 2.5cm。用生理盐水冲洗伤口后，将 Adv-hBMP-2/MC3T3-E1 纳米-HA 复合人工骨、MC3T3-E1 纳米-HA 复合人工骨或纳米-HA 人工骨分别植入不同组。本研究制备的人工骨支架具有更强的修复骨缺损的能力，是骨缺损临床治疗的一种有前途的方法。
