Biophysics Program, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109, USA.
Current Address: Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, 1000, Ljubljana, Slovenia.
Biomol NMR Assign. 2021 Oct;15(2):479-490. doi: 10.1007/s12104-021-10049-0. Epub 2021 Aug 27.
RNAs play myriad functional and regulatory roles in the cell. Despite their significance, three-dimensional structure elucidation of RNA molecules lags significantly behind that of proteins. NMR-based studies are often rate-limited by the assignment of chemical shifts. Automation of the chemical shift assignment process can greatly facilitate structural studies, however, accurate chemical shift predictions rely on a robust and complete chemical shift database for training. We searched the Biological Magnetic Resonance Data Bank (BMRB) to identify sequences that had no (or limited) chemical shift information. Here, we report the chemical shift assignments for 12 RNA hairpins designed specifically to help populate the BMRB.
RNAs 在细胞中发挥着多种功能和调节作用。尽管它们很重要，但 RNA 分子的三维结构解析远远落后于蛋白质。基于 NMR 的研究通常受到化学位移分配的限制。化学位移分配过程的自动化可以极大地促进结构研究，然而，准确的化学位移预测依赖于一个强大而完整的化学位移数据库进行训练。我们在生物磁共振数据银行（BMRB）中搜索了没有（或有限）化学位移信息的序列。在这里，我们报告了 12 个 RNA 发夹的化学位移分配，这些发夹是专门设计用来帮助 BMRB 填充的。
