Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China.
Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China.
Dev Comp Immunol. 2022 Jan;126:104242. doi: 10.1016/j.dci.2021.104242. Epub 2021 Aug 25.
Although, in mammals, the Krüppel-like transcription factor 13 (KLF13) plays an essential role in cell proliferation, survival, differentiation, apoptosis, tumorigenesis, immune regulation, and inflammation, its role in penaeid shrimp is unclear. In the current study, we characterized a KLF13 homolog in Penaeus vannamei (PvKLF13), with full-length cDNA of 1677 bp and 1068 bp open reading frame (ORF) encoding a putative protein of 355 amino acids, which contains three ZnF_C2H2 domains. Sequence and phylogenetic analysis revealed that PvKLF13 shares a close evolutionary relationship with KLF13 from invertebrates. Transcript levels of PvKLF13 were ubiquitously expressed in shrimp and induced in hemocytes upon challenge with Vibrio parahaemolyticus, Streptococcus iniae, and white spot syndrome virus (WSSV), suggesting the involvement of PvKLF13 in shrimp immune response to pathogens. Besides, knockdown of PvKLF13 decreased hemocytes apoptosis in terms of increased expression of pro-survival PvBcl-2, but decreased expression of pro-apoptotic PvBax and PvCytochrome C, coupled with high PvCaspase3/7 activity, especially upon V. parahaemolyticus challenge. The findings here indicate the involvement of PvKLF13 in apoptotic cell clearance as an essential part of shrimp innate immune response to pathogens.
虽然在哺乳动物中，Krüppel 样转录因子 13（KLF13）在细胞增殖、存活、分化、凋亡、肿瘤发生、免疫调节和炎症中发挥着重要作用，但它在对虾中的作用尚不清楚。在本研究中，我们对凡纳滨对虾（Penaeus vannamei）中的 KLF13 同源物（PvKLF13）进行了特征描述，其 cDNA 全长 1677bp，开放阅读框（ORF）长 1068bp，编码一个由 355 个氨基酸组成的假定蛋白，其中包含三个 ZnF_C2H2 结构域。序列和系统发育分析表明，PvKLF13 与无脊椎动物的 KLF13 具有密切的进化关系。PvKLF13 在虾体中广泛表达，并在受到副溶血弧菌、鳗弧菌和白斑综合征病毒（WSSV）的刺激后在血细胞中诱导表达，表明 PvKLF13 参与了虾对病原体的免疫反应。此外，PvKLF13 的敲低导致血细胞凋亡增加，表现为抗凋亡基因 PvBcl-2 表达增加，而促凋亡基因 PvBax 和 PvCytochrome C 表达减少，同时 PvCaspase3/7 活性升高，特别是在受到副溶血弧菌刺激时。这些发现表明 PvKLF13 参与了细胞凋亡清除，作为虾先天免疫反应清除病原体的一个重要组成部分。
