State Key Laboratory of Food Nutrition & Safety, Tianjin University of Science & Technology, Tianjin 300457, China; Key Laboratory of Industrial Fermentation Microbiology, (Ministry of Education), Tianjin University of Science & Technology, Tianjin 300457, China.
State Key Laboratory of Food Nutrition & Safety, Tianjin University of Science & Technology, Tianjin 300457, China; Key Laboratory of Industrial Fermentation Microbiology, (Ministry of Education), Tianjin University of Science & Technology, Tianjin 300457, China.
Int J Biol Macromol. 2021 Oct 31;189:483-493. doi: 10.1016/j.ijbiomac.2021.08.157. Epub 2021 Aug 24.
Hydrogels with pH sensitivity and stable mechanical and antibacterial properties have many desirable qualities and broad applications. A hydrogel based on bacterial cellulose and chitosan, impregnated with silver sulfadiazine (<1% w/w), was prepared using glutaraldehyde as the crosslinking agent. The presence of SSd was confirmed by Fourier transform infrared spectroscopy. Micropore size, swelling ratio, pH- sensitivity, and gram positive and negative antibacterial properties were studied by disk diffusion and colony forming unit. X-ray diffraction confirmed the presence of amorphous and crystalline regions in the hydrogel matrix following addition of SSd. The elemental composition, morphology, and mechanical properties of the hydrogels were characterized. Incorporation of SSd into bacterial cellulose-chitosan hydrogels significantly improved their mechanical and antibacterial properties. The antibacterial activity against E. coli and S. aureus was evaluated and SSd-BC/Ch hydrogels are more toxic to S. aureus than to E. coli. We use FESEM to observe bacterial morphology before and after exposure to SSd-BC/Ch hydrogels. The BacLight LIVE/DEAD membrane permeability kit is used to evaluate the membrane permeability of bacteria. These antibacterial hydrogels have many promising applications in food packaging, tissue engineering, drug delivery, clinical, biotechnological, and biomedical fields.
具有 pH 敏感性和稳定机械性能及抗菌性能的水凝胶具有许多理想的特性和广泛的应用。本文制备了一种基于细菌纤维素和壳聚糖的水凝胶，用戊二醛作为交联剂，将磺胺嘧啶银（<1%w/w）浸渍其中。傅里叶变换红外光谱证实了磺胺嘧啶银的存在。通过圆盘扩散和集落形成单位研究了微孔大小、溶胀比、pH 敏感性以及革兰氏阳性和阴性抗菌性能。X 射线衍射证实了磺胺嘧啶银加入后水凝胶基质中存在无定形和结晶区。对水凝胶的元素组成、形态和力学性能进行了表征。磺胺嘧啶银的加入显著提高了细菌纤维素-壳聚糖水凝胶的力学性能和抗菌性能。评价了磺胺嘧啶银-细菌纤维素/壳聚糖水凝胶对大肠杆菌和金黄色葡萄球菌的抗菌活性，结果表明磺胺嘧啶银-细菌纤维素/壳聚糖水凝胶对金黄色葡萄球菌的毒性大于大肠杆菌。我们使用 FESEM 观察磺胺嘧啶银-细菌纤维素/壳聚糖水凝胶处理前后细菌的形态。使用 BacLight LIVE/DEAD 膜通透性试剂盒评估细菌的膜通透性。这些抗菌水凝胶在食品包装、组织工程、药物输送、临床、生物技术和生物医学领域具有许多有前途的应用。
