在5埃分辨率下对天花粉蛋白正交晶型进行的X射线分析。
An X-ray analysis of the orthorhombic crystal form of trichosanthin at 5 angstrom.
作者信息
Ma X Q, Wang Y P, Wang J H
机构信息
Institute of Biophysics, Academia Sinica, Beijing.
出版信息
Sci Sin B. 1987 Jul;30(7):692-7.
Trichosanthin crystals grown at pH 5.4 are orthorhombic P212121 with cell dimension a = 38.23 angstrom, b = 76.18 angstrom and c = 79.12 angstrom. Each asymmetric unit contains one protein molecule of 234 amino acid residues (M. W. 26,000). The structure has been solved at 5 angstrom resolution by conventional MIR method with two heavy-atom derivatives. The preliminary result suggests that the protein molecule in acidic solution does not have dramatic conformational change, compared with that in alkali solution.
在pH 5.4条件下生长的天花粉蛋白晶体属于正交晶系P212121，晶胞参数为a = 38.23埃，b = 76.18埃，c = 79.12埃。每个不对称单元包含一个由234个氨基酸残基组成的蛋白质分子（分子量为26,000）。该结构已通过传统的多波长反常散射法（MIR），利用两种重原子衍生物在5埃分辨率下解析出来。初步结果表明，与在碱性溶液中的情况相比，酸性溶液中的蛋白质分子没有显著的构象变化。
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