Tedesco Perla, Saraiva Marcia, Sandoval-Sierra Jose Vladimir, Fioravanti Maria Letizia, Morandi Benedetto, Dieguez-Uribeondo Javier, van West Pieter, Galuppi Roberta
Department of Veterinary Medical Sciences, Alma Mater Studiorum-University of Bologna, Ozzano Emilia, 40064 Bologna, Italy.
Aberdeen Oomycete Laboratory, International Centre for Aquaculture Research and Development (ICARD), Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK.
Pathogens. 2021 Jul 22;10(8):926. doi: 10.3390/pathogens10080926.
infections are among the main parasitic diseases affecting farmed salmonids. The distribution and potential transfer of spp. between farms and the natural environment has been scarcely investigated. Therefore, this work aimed to study the diversity and abundance of oomycete species in salmonid farms, tributary water, and effluent water systems. Four trout farms in Italy and two Atlantic salmon farms in Scotland were considered. In Italian farms, 532 isolates of oomycetes were obtained from fish and water, at upstream, inside, and downstream the farms. In Scottish farms, 201 oomycetes isolates were obtained from water outside the farm and from fish and water inside the farming units. Isolates were identified to the species level through amplification and sequencing of the ITS rDNA region. In Italy, was significantly more present in farmed than in wild fish, while in water it was more frequently isolated from the wild, particularly in effluent systems, not associated with more frequent isolation of in wild fish downstream the farm. In Scotland, was the most prevalent species isolated from fish, while isolates from water were mostly spp. with few isolates from upstream and downstream the farms.
感染是影响养殖鲑科鱼类的主要寄生虫病之一。关于某些物种在养殖场与自然环境之间的分布及潜在转移情况,鲜有研究。因此,本研究旨在探讨鲑科鱼类养殖场、支流及废水系统中卵菌物种的多样性和丰度。研究涉及意大利的四个鳟鱼养殖场和苏格兰的两个大西洋鲑鱼养殖场。在意大利的养殖场,从养殖场上游、场内及下游的鱼和水中获取了532株卵菌分离株。在苏格兰的养殖场,从养殖场外的水以及养殖单元内的鱼和水中获取了201株卵菌分离株。通过对ITS rDNA区域进行扩增和测序,将分离株鉴定到物种水平。在意大利,养殖鱼类中该物种的存在显著多于野生鱼类,而在水中,野生环境中分离到该物种的频率更高,尤其是在废水系统中,且这与养殖场下游野生鱼类中该物种的更频繁分离无关。在苏格兰,该物种是从鱼类中分离出的最常见物种,而从水中分离出的菌株大多为其他物种,养殖场上下游的该物种分离株较少。