Development of multiplex PCR assay for species-specific detection and identification of .

is the most important pathogen under the genus, which causes devastating oomycete diseases in freshwater fish. At present, the most common molecular method for identification of species is sequencing of ribosomal DNA internal transcribed spacer (rDNA-ITS) region. In this study, a highly sensitive multiplex PCR targeting rDNA-ITS region and a hypothetical protein gene was developed using two sets of primer pair. In this PCR, two amplicons of different size of 750 bp and 365 bp are produced only in case of while other species had single amplicon. This protocol could also differentiate species from other fungus based on the size of rDNA-ITS region. The protocol does not require sequencing and can identify in a single reaction. Therefore, the multiplex PCR developed in this study may prove to be an easier, faster and cheaper molecular method for identification of