裸鼹鼠cathelicidins的抗病毒活性及其对人单纯疱疹病毒1型的作用特性

Characterisation of Antiviral Activity of Cathelicidins from Naked Mole Rat and on Human Herpes Simplex Virus 1.

作者信息

Damour Alexia, Garcia Magali, Cho Hye-Sun, Larivière Andy, Lévêque Nicolas, Park Chankyu, Bodet Charles

机构信息

Laboratoire Inflammation Tissus Epithéliaux et Cytokines (LITEC EA 4331), Université de Poitiers, CEDEX 9, 86073 Poitiers, France.

Laboratoire de Virologie et Mycobactériologie, CHU de Poitiers, 86021 Poitiers, France.

出版信息

Pharmaceuticals (Basel). 2021 Jul 24;14(8):715. doi: 10.3390/ph14080715.

DOI:10.3390/ph14080715

PMID:34451812

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8398704/

Abstract

Hg-CATH and Pb-CATH4 are cathelicidins from and that have been previously identified as potent antibacterial peptides. However, their antiviral properties were not previously investigated. In this study, their activity against the herpes simplex virus (HSV)-1 was evaluated during primary human keratinocyte infection. Both of them significantly reduced HSV-1 DNA replication and production of infectious viral particles in keratinocytes at noncytotoxic concentrations, with the stronger activity of Pb-CATH4. These peptides did not show direct virucidal activity and did not exhibit significant immunomodulatory properties, except for Pb-CATH4, which exerted a moderate proinflammatory action. All in all, our results suggest that Hg-CATH and Pb-CATH4 could be potent candidates for the development of new therapies against HSV-1.

摘要

Hg-CATH和Pb-CATH4是分别来自[具体来源1]和[具体来源2]的cathelicidin，此前已被鉴定为强效抗菌肽。然而，它们的抗病毒特性此前未被研究。在本研究中，对它们在原代人角质形成细胞感染期间对单纯疱疹病毒1型（HSV-1）的活性进行了评估。在无细胞毒性浓度下，它们二者均显著降低了角质形成细胞中HSV-1的DNA复制及感染性病毒颗粒的产生，其中Pb-CATH4的活性更强。这些肽未表现出直接的杀病毒活性，且除Pb-CATH4具有适度的促炎作用外，未表现出显著的免疫调节特性。总而言之，我们的结果表明，Hg-CATH和Pb-CATH4可能是开发抗HSV-1新疗法的有力候选物。