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重组蛋白和GM-CSF DNA疫苗引发的与保护相关的3型细胞介导免疫反应

Protection-Related Type 3 Cell-Mediated Immune Response Elicited by Recombinant Proteins and GM-CSF DNA Vaccine.

作者信息

Santos Kamila R, Souza Fernando N, Ramos-Sanchez Eduardo M, Batista Camila F, Reis Luiza C, Fotoran Wesley F, Heinemann Marcos B, Goto Hiro, Gidlund Magnus, Cunha Adriano F, Faria Angélica Rosa, Andrade Hélida M, Lage Andrey P, Cerqueira Mônica M O P, Della Libera Alice M M P

机构信息

Veterinary Clinical Immunology Research Group, Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil.

Programa de Pós-Graduação em Ciência Animal, Universidade Federal da Paraíba, Areia 58397-000, Brazil.

出版信息

Vaccines (Basel). 2021 Aug 13;9(8):899. doi: 10.3390/vaccines9080899.

DOI:10.3390/vaccines9080899
PMID:34452024
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8402413/
Abstract

mastitis remains a major challenge for dairy farming. Here, 24 mice were immunized and divided into four groups: G1: control; G2: Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) DNA vaccine; G3: F0F1 ATP synthase subunit α (SAS), succinyl-diaminopimelate (SDD), and cysteinyl-tRNA synthetase (CTS) recombinant proteins; and G4: SAS+SDD+CTS plus GM-CSF DNA vaccine. The lymphocyte subpopulations, and the intracellular interleukin-17A (IL-17A) and interferon-γ production in the draining lymph node cells were immunophenotyped by flow cytometry. The immunophenotyping and lymphocyte proliferation was determined in spleen cells cultured with and without stimulus. Immunization with recombinant proteins generated memory cells in draining lymph nodes. Immunization with the three recombinant proteins plus GM-CSF DNA led to an increase in the percentage of IL-17A cells among overall CD44 (memory), T CD4, CD4 T CD44 CD27, γδ TCR, γδ TCR CD44 CD27, and TCRVγ4 cells. Vaccination with recombinant proteins associated with GM-CSF DNA vaccine downregulated T2 immunity. Immunization with the three recombinant proteins plus the GM-CSF DNA led to a proliferation of overall memory T, CD4, and CD4 TEM cells upon stimulus. This approach fostered type 3 immunity, suggesting the development of a protective immune response against .

摘要

乳腺炎仍然是奶牛养殖面临的一项重大挑战。在此，对24只小鼠进行免疫并分为四组：G1：对照组；G2：粒细胞巨噬细胞集落刺激因子（GM-CSF）DNA疫苗组；G3：F0F1 ATP合酶亚基α（SAS）、琥珀酰二氨基庚二酸（SDD）和半胱氨酰tRNA合成酶（CTS）重组蛋白组；以及G4：SAS+SDD+CTS加GM-CSF DNA疫苗组。通过流式细胞术对引流淋巴结细胞中的淋巴细胞亚群以及细胞内白细胞介素-17A（IL-17A）和干扰素-γ的产生进行免疫表型分析。在有或无刺激的情况下培养脾细胞，测定其免疫表型分析和淋巴细胞增殖情况。用重组蛋白免疫可在引流淋巴结中产生记忆细胞。用三种重组蛋白加GM-CSF DNA进行免疫导致在总的CD44（记忆）、T CD4、CD4 T CD44 CD27、γδ TCR、γδ TCR CD44 CD27和TCRVγ4细胞中IL-17A细胞百分比增加。用与GM-CSF DNA疫苗相关的重组蛋白进行疫苗接种可下调T2免疫。用三种重组蛋白加GM-CSF DNA进行免疫导致在受到刺激时总的记忆T、CD4和CD4 TEM细胞增殖。这种方法促进了3型免疫，表明针对……产生了保护性免疫反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/8402413/bfd1bd71e301/vaccines-09-00899-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/8402413/1e97d70a24d8/vaccines-09-00899-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/8402413/77f573305c4e/vaccines-09-00899-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/8402413/52ab49c9be41/vaccines-09-00899-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/8402413/0fc4cefdb2f8/vaccines-09-00899-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/8402413/bfd1bd71e301/vaccines-09-00899-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/8402413/1e97d70a24d8/vaccines-09-00899-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/8402413/77f573305c4e/vaccines-09-00899-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/8402413/52ab49c9be41/vaccines-09-00899-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/8402413/0fc4cefdb2f8/vaccines-09-00899-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc3f/8402413/bfd1bd71e301/vaccines-09-00899-g005.jpg

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引用本文的文献

[1]
Cure-Associated Antigens Elicit Type 3 Immune Memory T Cells.

Antibiotics (Basel). 2022-12-16

本文引用的文献

[1]
T Cell Immunity and the Quest for Protective Vaccines against Infection.

Microorganisms. 2020-12-6

[2]
Molecular Typing and Antimicrobial Susceptibility Profile of Isolates Recovered from Bovine Mastitis and Nasal Samples.

Animals (Basel). 2020-11-18

[3]
Type 3 immunity: a perspective for the defense of the mammary gland against infections.

Vet Res. 2020-10-15

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Staphylococcus aureus ATP Synthase Promotes Biofilm Persistence by Influencing Innate Immunity.

mBio. 2020-9-8

[5]
Activation of Human Vδ2 γδ T Cells by Promotes Enhanced Anti-Staphylococcal Adaptive Immunity.

J Immunol. 2020-8-15

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Inhibition of the ATP synthase sensitizes Staphylococcus aureus towards human antimicrobial peptides.

Sci Rep. 2020-7-9

[7]
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Front Immunol. 2020

[8]
Rapid and Broad Immune Efficacy of a Recombinant Five-Antigen Vaccine against Infection in Animal Models.

Vaccines (Basel). 2020-3-18

[9]
Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine.

J Dairy Sci. 2020-2-26

[10]
Development of a vaccine against Staphylococcus aureus invasive infections: Evidence based on human immunity, genetics and bacterial evasion mechanisms.

FEMS Microbiol Rev. 2020-1-1

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