Santos Renata P, Souza Fernando N, Oliveira Ana Claudia D, de Souza Filho Antônio F, Aizawa Juliana, Moreno Luisa Z, da Cunha Adriano F, Cortez Adriana, Della Libera Alice M M P, Heinemann Marcos B, Cerqueira Mônica M O P
Departamento de Inspeção e Produtos de Origem Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte 31270-901, Brazil.
Departamento de Clínica Médica, Faculdade de Medicina e Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, Brazil.
Animals (Basel). 2020 Nov 18;10(11):2143. doi: 10.3390/ani10112143.
In the present study, we aimed to determine the antimicrobial resistance and molecular typing of recovered from transient and persistent intramammary infections and nares/muzzles in dairy cows. We investigated the antimicrobial resistance of 189 strains using a broad antimicrobial susceptibility profile. Furthermore, 107 isolates were strain-typed using staphylococcal protein-A (spa) typing. A large proportion of strains exhibited multidrug resistance to antimicrobials, including resistance to critically important antimicrobials, although no methicillin-resistant strains were found. Our study did not strengthen the idea that extramammary niches (i.e., nares/muzzles) are an important source of for bovine mastitis. A discrepancy in the antimicrobial resistance between strains isolated from nares/muzzles and milk samples was observed. Furthermore, isolates from transient and persistent intramammary infections (IMIs) did not differ by spa typing, suggesting that the persistence of bovine IMIs was determined by cow factors. Thus, the high level of multidrug-resistant found in the two herds, considered together with the predominance of a well udder-adapted strain, may contribute to our knowledge of the history of the high prevalence of mastitis caused by , which is of great concern for animal and public health.
在本研究中，我们旨在确定从奶牛的短暂性和持续性乳房内感染以及鼻孔/口鼻部分离出的金黄色葡萄球菌的抗菌药物耐药性和分子分型。我们使用广泛的抗菌药物敏感性谱研究了189株金黄色葡萄球菌的抗菌药物耐药性。此外，使用葡萄球菌蛋白A（spa）分型对107株分离株进行了菌株分型。尽管未发现耐甲氧西林金黄色葡萄球菌菌株，但很大一部分菌株对多种抗菌药物表现出多重耐药性，包括对极为重要的抗菌药物耐药。我们的研究并未强化乳腺外生态位（即鼻孔/口鼻部）是牛乳腺炎重要金黄色葡萄球菌来源的观点。观察到从鼻孔/口鼻部和牛奶样本中分离出的金黄色葡萄球菌菌株在抗菌药物耐药性方面存在差异。此外，从短暂性和持续性乳房内感染（IMIs）中分离出的金黄色葡萄球菌菌株通过spa分型没有差异，这表明牛IMIs的持续性是由奶牛因素决定的。因此，在这两个牛群中发现的高水平多重耐药金黄色葡萄球菌，再加上适应乳房的金黄色葡萄球菌菌株占主导地位，可能有助于我们了解由金黄色葡萄球菌引起的乳腺炎高流行率的历史，这对动物和公共卫生都至关重要。
