Centre for Advanced Macromolecular Design (CAMD) and Australian Centre for NanoMedicine (ACN), School of Chemical Engineering, UNSW Australia, Sydney, NSW, 2052, Australia.
Angew Chem Int Ed Engl. 2021 Nov 2;60(45):24248-24256. doi: 10.1002/anie.202110672. Epub 2021 Oct 4.
This work presents the synthesis of a novel photosensitive acrylate monomer for use as both a self-catalyst in the photoinduced electron/energy transfer-reversible addition fragmentation chain transfer (PET-RAFT) polymerisation process and a photosensitiser (PS) for antibacterial applications. Hydrophilic, cationic, and antimicrobial formulations are explored to compare the antibacterial effects between charged and non-charged polymers. Covalent attachment of the catalyst to well-defined linear polymer chains has no effect on polymerisation control or singlet oxygen generation. The addition of the PS to polymers provides activity against S. aureus for all polymer formulations, resulting in up to a 99.99999 % killing efficacy in 30 min. Antimicrobial peptide mimetic polymers previously active against P. aeruginosa, but not S. aureus, gain significant bactericidal activity against S. aureus through the inclusion of PS groups, with 99.998 % killing efficiency after 30 min incubation with light. Thus, a broader spectrum of antimicrobial activity is achieved using two distinct mechanisms of bactericidal activity via the incorporation of a photosensitiser monomer into an antimicrobial polymer.
这项工作提出了一种新型光敏感丙烯酸酯单体的合成方法，可同时用作光诱导电子/能量转移-可逆加成-断裂链转移（PET-RAFT）聚合过程中的自催化剂和用于抗菌应用的光增敏剂（PS）。探索了亲水、阳离子和抗菌配方，以比较带电荷和不带电荷聚合物之间的抗菌效果。将催化剂共价连接到结构明确的线性聚合物链上对聚合控制或单线态氧生成没有影响。将 PS 添加到聚合物中为所有聚合物配方提供了针对金黄色葡萄球菌的活性，导致在 30 分钟内达到高达 99.99999%的杀伤效力。以前对铜绿假单胞菌有活性但对金黄色葡萄球菌没有活性的抗菌肽模拟聚合物通过包含 PS 基团获得了针对金黄色葡萄球菌的显著杀菌活性，在 30 分钟的光照孵育后，杀菌效率达到 99.998%。因此，通过将光敏剂单体掺入抗菌聚合物中，使用两种不同的杀菌机制实现了更广泛的抗菌活性。
