College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, China.
College of National Medicine, Guizhou Minzu University, Guiyang, 550025, China.
Comb Chem High Throughput Screen. 2022;25(10):1756-1766. doi: 10.2174/1386207324666210827112233.
Musa basjoo pseudostem juice (MBSJ) is a well-known Chinese medicine, and Miao people use MBSJ to treat diabetes. In this work, the active ingredients and molecular mechanism of MBSJ against diabetes were explored.
Anti-diabetic activity of MBSJ was evaluated using diabetic rats, and then the ingredients in the small-polar parts of MBSJ were analyzed by gas chromatography-mass spectrometer (GC-MS). Targets were obtained from several databases to develop the "ingredienttarget- disease" network by Cytoscape. A collaborative analysis was carried out using the tools in Cytoscape and R packages, and molecular docking was also performed.
MBSJ improved the oral glucose tolerance and insulin tolerance, and reduced fasting blood glucose, glycosylated hemoglobin, total cholesterol, triglyceride, and low-density lipoprotein levels in the serum of diabetic rats. 13 potential compounds were identified by GC-MS for subsequent analysis, including Dibutyl phthalate, Oleamide, Stigmasterol, Stigmast-4-en-3-one, etc. The anti-diabetic effect of MBSJ was related to multiple signaling pathways, including Neuroactive ligand-receptor interaction, Phospholipase D signaling pathway, Endocrine resistance, Rap1 signaling pathway, EGFR tyrosine kinase inhibitor resistance, etc. Molecular docking at least partially verified the screening results of network pharmacology.
MBSJ had good anti-diabetic activity. The small-polar parts of MBSJ were rich in anti-diabetic active ingredients. Furthermore, the analysis results showed that the anti-diabetic effect of the small-polar parts of MBSJ may be the result of multiple components, multiple targets, and multiple pathways. The current research results can provide important support for studying the active ingredients and exploring the underlying mechanism of MBSJ against diabetes.
芭蕉假茎汁（MBSJ）是一种著名的中药，苗族人用 MBSJ 治疗糖尿病。本工作旨在探讨 MBSJ 抗糖尿病的活性成分和分子机制。
采用糖尿病大鼠评价 MBSJ 的抗糖尿病活性，然后采用气相色谱-质谱联用（GC-MS）分析 MBSJ 小极性部位的成分。从多个数据库中获取靶点，通过 Cytoscape 构建“成分-靶点-疾病”网络。利用 Cytoscape 中的工具和 R 包进行协同分析，并进行分子对接。
MBSJ 改善了糖尿病大鼠的口服葡萄糖耐量和胰岛素耐量，降低了血清空腹血糖、糖化血红蛋白、总胆固醇、甘油三酯和低密度脂蛋白水平。GC-MS 鉴定出 13 种潜在化合物，用于后续分析，包括邻苯二甲酸二丁酯、油酰胺、豆甾醇、豆甾-4-烯-3-酮等。MBSJ 的抗糖尿病作用与多种信号通路有关，包括神经活性配体-受体相互作用、磷酯酶 D 信号通路、内分泌抵抗、Rap1 信号通路、表皮生长因子受体酪氨酸激酶抑制剂耐药等。分子对接至少部分验证了网络药理学的筛选结果。
MBSJ 具有良好的抗糖尿病活性。MBSJ 的小极性部位富含抗糖尿病活性成分。此外，分析结果表明，MBSJ 小极性部位的抗糖尿病作用可能是多种成分、多种靶点和多种途径共同作用的结果。本研究结果可为研究 MBSJ 抗糖尿病的活性成分和探索其作用机制提供重要支持。
