N-甲基腺苷RNA修饰水平的斑点印迹分析
Dot Blot Analysis of N-methyladenosine RNA Modification Levels.
作者信息
Shen Lisha, Liang Zhe, Yu Hao
机构信息
Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore.
出版信息
Bio Protoc. 2017 Jan 5;7(1):e2095. doi: 10.21769/BioProtoc.2095.
Abstract
N-methyladenosine (mA) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). The total amount of mA can be detected by several methods, such as dot blot analysis using specific mA antibodies and quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) ( Fu , 2014 ; Shen , 2016 ). Here we describe the method for fast detection of total mA levels in mRNA by dot blot analysis using a specific mA antibody.
摘要
N6-甲基腺苷（mA）是真核生物信使核糖核酸（mRNA）中最普遍的内部修饰。mA的总量可以通过多种方法检测，如使用特异性mA抗体的斑点印迹分析和定量液相色谱-串联质谱法（LC-MS/MS）（傅，2014；沈，2016）。本文我们描述了使用特异性mA抗体通过斑点印迹分析快速检测mRNA中总mA水平的方法。
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