Das Priyanka, Sahoo Santilata
Division of Plant Biology, Bose Institute, Kolkata, India.
Department of Botany, Utkal University, Bhubaneswar, India.
Bio Protoc. 2017 Jan 20;7(2):e2104. doi: 10.21769/BioProtoc.2104.
Floral initiation and development in the angiosperms is the essential step on which the yield of the plant depends. Sometimes external climate or any abiotic stress hinders the floral initiation and ultimately affect the plant yield. Hence, floral induction and development can help to overcome the external climatic factor. Furthermore, the protocol for floral induction in the parasitic angiosperm like has not been reported yet. We have standardized the protocol for floral induction in the parasitic plant . In this study it is established that MMS (modified Murashige Skoog) media supplemented with 2 mg L NAA (naphthalene acetic acid, plant growth regulator) and 2 mg L 2,4-D (2,4-dichlorophenoxy acetic acid, plant growth regulator) supported floral induction after shooting . Furthermore, we found that MMS media supplemented with 2 mg L 2,4-D rapidly induced floral buds directly from the nodal explants without any shoot elongation. This protocol will help the researcher to induce flower in the other angiosperm plants along with .